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Literature summary for 1.17.1.4 extracted from

  • Nakamura, M.; Yamazaki, I.
    Preparation of bovine milk xanthine oxidase as a dehydrogenase form (1982), J. Biochem., 92, 1279-1286.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
DTT recovery from oxidase to dehydrogenase type Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
lipid droplet fat-globule, milk Bos taurus 5811
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xanthine + NAD+ + H2O Bos taurus conversion of dehydrogenase to oxidase type due to oxidation of sulfhydryl groups by molecular oxygen, dehydrogenase activity recovered by treatment with dithiothreitol urate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Oxidation Stability

Oxidation Stability Organism
conversion of dehydrogenase to oxidase type under aerobic conditions Bos taurus

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 2 types: dehydrogenase type, chromatography techniques, oxidase type: affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
milk only oxidase type in stored milk, 94% NAD+-dependent dehydrogenase type in fresh milk Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xanthine + NAD+ + H2O conversion of dehydrogenase to oxidase type due to oxidation of sulfhydryl groups by molecular oxygen, dehydrogenase activity recovered by treatment with dithiothreitol Bos taurus urate + NADH
-
?
xanthine + p-benzoquinone + H2O electron acceptor p-benzoquinone for both dehydrogenase and oxidase types Bos taurus p-benzosemiquinone + urate
-
?
xanthine + p-benzoquinone + H2O electron donor only for oxidase type Bos taurus hypoxanthine + hydroquinone + ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2
-
dehydrogenase type stable under anaerobic conditions Bos taurus
38
-
dehydrogenase type unstable, change to oxidase type inhibited under anaerobic conditions Bos taurus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bos taurus
NADH reduces xanthine oxidase to reductase activity Bos taurus