Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | recovery from oxidase to dehydrogenase type | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lipid droplet | fat-globule, milk | Bos taurus | 5811 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + NAD+ + H2O | Bos taurus | conversion of dehydrogenase to oxidase type due to oxidation of sulfhydryl groups by molecular oxygen, dehydrogenase activity recovered by treatment with dithiothreitol | urate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
conversion of dehydrogenase to oxidase type under aerobic conditions | Bos taurus |
Purification (Comment) | Organism |
---|---|
to homogeneity, 2 types: dehydrogenase type, chromatography techniques, oxidase type: affinity chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
milk | only oxidase type in stored milk, 94% NAD+-dependent dehydrogenase type in fresh milk | Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + NAD+ + H2O | conversion of dehydrogenase to oxidase type due to oxidation of sulfhydryl groups by molecular oxygen, dehydrogenase activity recovered by treatment with dithiothreitol | Bos taurus | urate + NADH | - |
? | |
xanthine + p-benzoquinone + H2O | electron acceptor p-benzoquinone for both dehydrogenase and oxidase types | Bos taurus | p-benzosemiquinone + urate | - |
? | |
xanthine + p-benzoquinone + H2O | electron donor only for oxidase type | Bos taurus | hypoxanthine + hydroquinone + ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
2 | - |
dehydrogenase type stable under anaerobic conditions | Bos taurus |
38 | - |
dehydrogenase type unstable, change to oxidase type inhibited under anaerobic conditions | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bos taurus | |
NADH | reduces xanthine oxidase to reductase activity | Bos taurus |