Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | 26fold increase of cytochrome c reduction, 2fold increase of oxygen reduction | Veillonella atypica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-Azaxanthine | 50% inhibition of ferricyanide reduction in xanthine oxidation assay at 5 mM | Veillonella atypica | |
KCN | 10-50% inhibition of xanthine oxidation only in the presence of Tris or phosphate buffers from 0.01 to 0.1 M inhibitor concentration | Veillonella atypica | |
methanol | develops inhibition during course of catalysis, enhanced inhibition in the presence of ferricyanide in the oxygen-dependent oxidation of xanthine | Veillonella atypica | |
Urate | 50% inhibition of xanthine oxidation at 0.5 mM | Veillonella atypica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | - |
Veillonella atypica | |
Mo | - |
Veillonella atypica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
250000 | - |
sucrose density gradient centrifugation | Veillonella atypica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,8-dihydroxypurine + O2 | Veillonella atypica | - |
? + H2O2 | - |
? | |
hypoxanthine + O2 | Veillonella atypica | - |
xanthine + H2O2 | - |
? | |
hypoxanthine + urate | Veillonella atypica | oxygen-free assay | xanthine + 6,8-dihydroxypurine | - |
r | |
purine + NAD+ + H2O | Veillonella atypica | - |
8-hydroxypurine + NADH + H+ | - |
? | |
xanthine + O2 | Veillonella atypica | dismutation reaction | hypoxanthine + ? | - |
? | |
xanthine + O2 + H2O | Veillonella atypica | presence of ferredoxin enhances rate of oxygen reduction | urate + O2- + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Veillonella atypica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, precipitation and chromatography techniques | Veillonella atypica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Veillonella atypica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
- |
Veillonella atypica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dichloroindophenol + NADH + H+ | - |
Veillonella atypica | reduced 2,6-dichlorophenolindophenol + NAD+ | - |
r | |
6,8-dihydroxypurine + O2 | - |
Veillonella atypica | ? + H2O2 | - |
? | |
6,8-dihydroxypurine + O2 + H2O | - |
Veillonella atypica | ? + H2O2 | - |
? | |
9-methylhypoxanthine + O2 | - |
Veillonella atypica | 9-methylxanthine + H2O2 | - |
? | |
benzaldehyde + O2 | - |
Veillonella atypica | benzoate + H2O2 | - |
? | |
hypoxanthine + O2 | - |
Veillonella atypica | xanthine + H2O2 | - |
? | |
hypoxanthine + urate | oxygen-free assay | Veillonella atypica | xanthine + 6,8-dihydroxypurine | - |
r | |
purine + NAD+ + H2O | - |
Veillonella atypica | 8-hydroxypurine + NADH + H+ | - |
? | |
salicylaldehyde + O2 | - |
Veillonella atypica | salicylate + H2O2 | - |
r | |
xanthine + cytochrome c + H2O | presence of ferredoxin enhances cytochrom c reduction | Veillonella atypica | urate + reduced cytochrome c | - |
? | |
xanthine + ferredoxin + H2O | - |
Veillonella atypica | urate + reduced ferredoxin | - |
? | |
xanthine + ferricyanide + H2O | - |
Veillonella atypica | urate + ferrocyanide | - |
? | |
xanthine + methylene blue + H2O | - |
Veillonella atypica | urate + reduced methylene blue | - |
? | |
xanthine + myoglobin + H2O | - |
Veillonella atypica | urate + reduced myoglobin | - |
? | |
xanthine + nitroblue tetrazolium + H2O | - |
Veillonella atypica | urate + ? | - |
? | |
xanthine + O2 | dismutation reaction | Veillonella atypica | hypoxanthine + ? | - |
? | |
xanthine + O2 + H2O | presence of ferredoxin enhances rate of oxygen reduction | Veillonella atypica | urate + O2- + 2 H+ | - |
? | |
xanthine + phenazine methosulfate + H2O | - |
Veillonella atypica | urate + ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Salicylaldehyde | electron acceptor: oxygen | Veillonella atypica | |
0.7 | - |
purine | electron acceptor: oxygen | Veillonella atypica | |
0.717 | - |
cytochrome c | cosubstrate xanthine | Veillonella atypica | |
0.75 | - |
hypoxanthine | electron acceptor: oxygen | Veillonella atypica | |
0.8 | - |
oxygen | cosubstrate xanthine | Veillonella atypica | |
2.5 | - |
xanthine | electron acceptor: oxygen | Veillonella atypica | |
5.67 | - |
2,6-dichlorophenolindophenol | cosubstrate xanthine | Veillonella atypica | |
9.55 | - |
Nitro blue tetrazolium | cosubstrate xanthine | Veillonella atypica | |
18.2 | - |
ferricyanide | cosubstrate xanthine | Veillonella atypica | |
20.2 | - |
Ferredoxin | cosubstrate xanthine | Veillonella atypica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
xanthine oxidation, presence of oxygen, two pH-optima | Veillonella atypica |
9.5 | - |
xanthine oxidation, presence of oxygen, two pH-optima | Veillonella atypica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
unstable below, stabilized and activated at higher pH values | Veillonella atypica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Veillonella atypica | |
additional information | molybdoironflavoprotein: 8 : 8 : 2 : 1.5 ratio of iron to sulfide to flavin to molybdenum | Veillonella atypica |