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Literature summary for 1.17.1.4 extracted from

  • Wagner, R.; Cammack, R.; Andreesen, J.R.
    Purification and characterization of xanthine dehydrogenase from Clostridium acidiurici grown in the presence of selenium (1984), Biochim. Biophys. Acta, 791, 63-74.
No PubMed abstract available

General Stability

General Stability Organism
complete loss of activity when stored below 0°C Gottschalkia acidurici

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
methyl viologen electron donor: xanthine Gottschalkia acidurici
1.35
-
xanthine electron acceptor: methyl viologen Gottschalkia acidurici

Metals/Ions

Metals/Ions Comment Organism Structure
Fe non-heme iron-sulfur groups, 7.7 g-atom Fe, 7.5 g- atom S2- per mol enzyme Gottschalkia acidurici
Iron-sulfur-center 2 Fe2-S2-clusters Gottschalkia acidurici
Mo
-
Gottschalkia acidurici
molybdenum-center 1.8 g-atom molybdenum per native molecule Gottschalkia acidurici
Se 0.13 g-atom selenium per native enzyme molecule Gottschalkia acidurici
Tungsten 0.12 g-atom tungsten per native enzyme molecule Gottschalkia acidurici

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE Gottschalkia acidurici
53000
-
1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE Gottschalkia acidurici
56000
-
1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE Gottschalkia acidurici
83000
-
1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE Gottschalkia acidurici
110000
-
1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE Gottschalkia acidurici
224000
-
gel filtration Gottschalkia acidurici

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xanthine + O2 + H2O Gottschalkia acidurici very low activity urate + O2- + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Gottschalkia acidurici
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques, preparative gel electrophoresis Gottschalkia acidurici

Source Tissue

Source Tissue Comment Organism Textmining
cell culture selenium is needed for xanthine dehydrogenase formation Gottschalkia acidurici
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
385
-
-
Gottschalkia acidurici

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxypyrazolo(3,4-d)pyrimidine + methyl viologen + H2O i.e. allopurinol Gottschalkia acidurici 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + reduced methyl viologen
-
?
4-hydroxypyrazolo(3,4-d)pyrimidine + methyl viologen + H2O 8% of the activity compared to xanthine Gottschalkia acidurici 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + reduced methyl viologen
-
?
5-aminoimidazol-4-carboxamide + methyl viologen + H2O 1.1% of the activity compared to xanthine Gottschalkia acidurici ? + reduced methyl viologen
-
?
6-mercaptopurine + methyl viologen + H2O 9.5% of the activity compared to xanthine Gottschalkia acidurici ? + reduced methyl viologen
-
?
hypoxanthine + methyl viologen + H2O 7% of the activity compared to xanthine Gottschalkia acidurici xanthine + reduced methyl viologen
-
?
purine + methyl viologen + H2O 2% of the activity compared to xanthine Gottschalkia acidurici ? + reduced methyl viologen
-
?
xanthine + 2,6-dichlorophenolindophenol + H2O 2.5% of the activity compared to methyl viologen as electron acceptor Gottschalkia acidurici urate + ?
-
?
xanthine + benzyl viologen + H2O 52% of the activity compared to methyl viologen as electron acceptor Gottschalkia acidurici urate + reduced benzyl viologen
-
?
xanthine + ferricyanide + H2O 99% of the activity compared to methyl viologen as electron acceptor Gottschalkia acidurici urate + ferrocyanide
-
?
xanthine + methyl viologen + H2O best substrates tested, no activity with NAD+ or NADP+, 40% of the activity in the reverse reaction Gottschalkia acidurici urate + reduced methyl viologen
-
r
xanthine + methylene blue + H2O 87% of the activity compared to methyl viologen as electron acceptor Gottschalkia acidurici urate + reduced methylene blue
-
?
xanthine + nitroblue tetrazolium + H2O 32% of the activity compared to methyl viologen as electron acceptor Gottschalkia acidurici urate + ?
-
?
xanthine + O2 + H2O very low activity Gottschalkia acidurici urate + O2- + 2 H+
-
?

Subunits

Subunits Comment Organism
pentamer 1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE Gottschalkia acidurici

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Gottschalkia acidurici

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
optimum value in the reverse reaction with urate and reduced methyl viologen Gottschalkia acidurici
8.5
-
optimum value for oxidation of xanthine and reduction of methyl viologen Gottschalkia acidurici

Cofactor

Cofactor Comment Organism Structure
FAD 1.69 mol FAD per mol enzyme Gottschalkia acidurici
additional information molybdoironflavoprotein: ratio of non-heme iron to acid-labile sulfur to FAD to molybdenum to tungsten to selenium is 7.7 : 7.5 : 1.7 : 1.8 : 0.12 : 0.13 Gottschalkia acidurici