Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-Azaadenine | competitive inhibition of xanthine oxidation, Ki: 0.25 mM | Chlamydomonas reinhardtii | |
acetaldehyde | inactivation | Chlamydomonas reinhardtii | |
adenine | competitive inhibition of xanthine oxidation, Ki: 0.13 mM | Chlamydomonas reinhardtii | |
hypoxanthine | inactivation of xanthine oxidase activity, not in the presence of NAD+ | Chlamydomonas reinhardtii | |
NADH | inactivation closely related to associated diaphorase activity | Chlamydomonas reinhardtii | |
NADPH | inactivation closely related to diaphorase activity | Chlamydomonas reinhardtii | |
Sodium dithionite | irreversible inactivation by reduction of xanthine dehydrogenase, no recovery after dithionite elimination | Chlamydomonas reinhardtii | |
xanthine | irreversible inactivation by reduction of xanthine dehydrogenase; irreversible inhibition of xanthine oxidase activity, adenine and 8-azaadenine protects against inactivation, ferricyanide partially protects against inactivation, no inactivation in the presence of NAD+ | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H2O | Chlamydomonas reinhardtii | - |
xanthine + NADH + H+ | - |
? | |
hypoxanthine + NADP+ + H2O | Chlamydomonas reinhardtii | - |
xanthine + NADPH | - |
? | |
xanthine + NAD+ + H2O | Chlamydomonas reinhardtii | - |
urate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography, preparative gel electrophoresis | Chlamydomonas reinhardtii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Chlamydomonas reinhardtii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.69 | - |
- |
Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-azaxanthine + NAD+ + H2O | very low activity | Chlamydomonas reinhardtii | 8-aza-urate + NADH | - |
? | |
acetaldehyde + NAD+ + H2O | very low activity | Chlamydomonas reinhardtii | acetate + NADH + H+ | - |
? | |
adenine + NAD+ + H2O | low activity | Chlamydomonas reinhardtii | ? + NADH | - |
? | |
hypoxanthine + NAD+ + H2O | - |
Chlamydomonas reinhardtii | xanthine + NADH + H+ | - |
? | |
hypoxanthine + NADP+ + H2O | - |
Chlamydomonas reinhardtii | xanthine + NADPH | - |
? | |
NADH + electron acceptor + H2O | electron acceptor: nitroblue tetrazolium | Chlamydomonas reinhardtii | NAD+ + reduced electron acceptor | - |
? | |
NADPH + nitroblue tetrazolium + H2O | diaphorase activity | Chlamydomonas reinhardtii | NADP+ + reduced nitroblue tetrazolium | - |
? | |
xanthine + NAD+ + H2O | - |
Chlamydomonas reinhardtii | urate + NADH | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Chlamydomonas reinhardtii | |
NADP+ | - |
Chlamydomonas reinhardtii |