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Literature summary for 1.17.1.4 extracted from

  • Xiang, Q.; Edmondson, D.E.
    Purification and characterization of a prokaryotic xanthine dehydrogenase from Comamonas acidovorans (1996), Biochemistry, 35, 5441-5450.
    View publication on PubMed
Search for more literature for 1.17.1.4

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxypyrazolo(3,4-d)pyrimidine i.e. allopurinol; rapid inactivation under anaerobic conditions at 0.1 mM Delftia acidovorans
KCN irreversible inactivation Delftia acidovorans
Sodium dithionite reduction of enzyme Delftia acidovorans
xanthine reduction of enzyme Delftia acidovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
 xanthine electron acceptor: NAD+ Delftia acidovorans
0.16
-
 NAD+ electron donor: xanthine Delftia acidovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Iron-sulfur-center 2 Fe2-S2-clusters Delftia acidovorans
molybdenum-center contains molybdopterin mononucleotide rather than molybdopterin dinucleotide Delftia acidovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 alpha1, x * 90000 + beta1, x * 60000, SDS-PAGE Delftia acidovorans
90000
-
 alpha1, x * 90000 + beta1, x * 60000, SDS-PAGE Delftia acidovorans
287000
-
 gel filtration Delftia acidovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xanthine + NAD+ + H2O Delftia acidovorans
-
 urate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Delftia acidovorans
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein 3.6 mol per alpha/beta-protomer Delftia acidovorans

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Delftia acidovorans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Delftia acidovorans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
-
 Delftia acidovorans

Storage Stability

Storage Stability Organism
-80°C, 6 months Delftia acidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + electron acceptor + H2O NADH diaphorase activity with several acceptors Delftia acidovorans NAD+ + reduced electron acceptor
-
 ?
xanthine + 2,6-dichlorophenolindophenol + H2O
-
 Delftia acidovorans urate + ?
-
 ?
xanthine + ferricyanide + H2O
-
 Delftia acidovorans urate + ferrocyanide
-
 ?
xanthine + NAD+ + H2O
-
 Delftia acidovorans urate + NADH
-
 ?
xanthine + nitroblue tetrazolium + H2O
-
 Delftia acidovorans urate + ?
-
 ?

Subunits

Subunits Comment Organism
? alpha1, x * 90000 + beta1, x * 60000, SDS-PAGE Delftia acidovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
120
-
 xanthine electron acceptor: NAD+ Delftia acidovorans

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Delftia acidovorans
additional information molybdoironflavoprotein: ratio of iron to FAD to molybdenum is 4 : 1 : 1 Delftia acidovorans
NAD+ physiological cofactor Delftia acidovorans