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Literature summary for 1.17.1.4 extracted from

  • Self, W.T.; Stadtman, T.C.
    Selenium-dependent metabolism of purines: A selenium-dependent purine hydroxylase and xanthine dehydrogenase were purified from Clostridium purinolyticum and characterized (2000), Proc. Natl. Acad. Sci. USA, 97, 7208-7213.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
KCN cyanolyzable selenium, 75% inhibition at 15 mM Gottschalkia purinilytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
xanthine electron acceptor: 2,6-dichlorophenolindophenol Gottschalkia purinilytica
0.5
-
hypoxanthine electron acceptor: 2,6-dichlorophenolindophenol Gottschalkia purinilytica
1
-
4-hydroxypyrazolo(3,4-d)pyrimidine electron acceptor: 2,6-dichlorophenolindophenol Gottschalkia purinilytica
1
-
4-hydroxypyrazolo(3,4-d)pyrimidine i.e. allopurinol Gottschalkia purinilytica

Metals/Ions

Metals/Ions Comment Organism Structure
Se
-
Gottschalkia purinilytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
alpha4, 4 * 80000 + beta4, 4 * 35000 + gamma4, 4 * 16000, SDS-PAGE Gottschalkia purinilytica
35000
-
alpha4, 4 * 80000 + beta4, 4 * 35000 + gamma4, 4 * 16000, SDS-PAGE Gottschalkia purinilytica
80000
-
alpha4, 4 * 80000 + beta4, 4 * 35000 + gamma4, 4 * 16000, SDS-PAGE Gottschalkia purinilytica
529000
-
gel filtration Gottschalkia purinilytica

Organism

Organism UniProt Comment Textmining
Gottschalkia purinilytica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Gottschalkia purinilytica

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Gottschalkia purinilytica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6
-
xanthine-2,6-dichlorophenolindophenol assay Gottschalkia purinilytica

Storage Stability

Storage Stability Organism
-70°C, 6 months Gottschalkia purinilytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methylxanthine + ferricyanide + H2O
-
Gottschalkia purinilytica 1-methylurate + ferrocyanide
-
?
2-OH-purine + ferricyanide + H2O
-
Gottschalkia purinilytica ? + ferrocyanide
-
?
4-hydroxypyrazolo(3,4-d)pyrimidine + ferricyanide + H2O i.e. allopurinol Gottschalkia purinilytica 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + ferrocyanide
-
?
hypoxanthine + ferricyanide
-
Gottschalkia purinilytica xanthine + ferrocyanide
-
?
xanthine + 2,6-dichlorophenolindophenol + H2O
-
Gottschalkia purinilytica urate + ?
-
?
xanthine + ferricyanide + H2O preferred substrates, does not act with NAD+ or NADP+ Gottschalkia purinilytica urate + ferrocyanide
-
?

Subunits

Subunits Comment Organism
dodecamer alpha4, 4 * 80000 + beta4, 4 * 35000 + gamma4, 4 * 16000, SDS-PAGE Gottschalkia purinilytica

Cofactor

Cofactor Comment Organism Structure
ferricyanide does not act with NAD+ or NADP+ Gottschalkia purinilytica
additional information
-
Gottschalkia purinilytica