Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCN | cyanolyzable selenium, 75% inhibition at 15 mM | Gottschalkia purinilytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
xanthine | electron acceptor: 2,6-dichlorophenolindophenol | Gottschalkia purinilytica | |
0.5 | - |
hypoxanthine | electron acceptor: 2,6-dichlorophenolindophenol | Gottschalkia purinilytica | |
1 | - |
4-hydroxypyrazolo(3,4-d)pyrimidine | electron acceptor: 2,6-dichlorophenolindophenol | Gottschalkia purinilytica | |
1 | - |
4-hydroxypyrazolo(3,4-d)pyrimidine | i.e. allopurinol | Gottschalkia purinilytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Se | - |
Gottschalkia purinilytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
alpha4, 4 * 80000 + beta4, 4 * 35000 + gamma4, 4 * 16000, SDS-PAGE | Gottschalkia purinilytica |
35000 | - |
alpha4, 4 * 80000 + beta4, 4 * 35000 + gamma4, 4 * 16000, SDS-PAGE | Gottschalkia purinilytica |
80000 | - |
alpha4, 4 * 80000 + beta4, 4 * 35000 + gamma4, 4 * 16000, SDS-PAGE | Gottschalkia purinilytica |
529000 | - |
gel filtration | Gottschalkia purinilytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gottschalkia purinilytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Gottschalkia purinilytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Gottschalkia purinilytica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.6 | - |
xanthine-2,6-dichlorophenolindophenol assay | Gottschalkia purinilytica |
Storage Stability | Organism |
---|---|
-70°C, 6 months | Gottschalkia purinilytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methylxanthine + ferricyanide + H2O | - |
Gottschalkia purinilytica | 1-methylurate + ferrocyanide | - |
? | |
2-OH-purine + ferricyanide + H2O | - |
Gottschalkia purinilytica | ? + ferrocyanide | - |
? | |
4-hydroxypyrazolo(3,4-d)pyrimidine + ferricyanide + H2O | i.e. allopurinol | Gottschalkia purinilytica | 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + ferrocyanide | - |
? | |
hypoxanthine + ferricyanide | - |
Gottschalkia purinilytica | xanthine + ferrocyanide | - |
? | |
xanthine + 2,6-dichlorophenolindophenol + H2O | - |
Gottschalkia purinilytica | urate + ? | - |
? | |
xanthine + ferricyanide + H2O | preferred substrates, does not act with NAD+ or NADP+ | Gottschalkia purinilytica | urate + ferrocyanide | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | alpha4, 4 * 80000 + beta4, 4 * 35000 + gamma4, 4 * 16000, SDS-PAGE | Gottschalkia purinilytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ferricyanide | does not act with NAD+ or NADP+ | Gottschalkia purinilytica | |
additional information | - |
Gottschalkia purinilytica |