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Literature summary for 1.17.1.4 extracted from

  • Parschat, K.; Canne, C.; Huttermann, J.; Kappl, R.; Fetzner, S.
    Xanthine dehydrogenase from Pseudomonas putida 86: Specificity, oxidation-reduction potentials of its redox-active centers, and first EPR characterization (2001), Biochim. Biophys. Acta, 1544, 151-165.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains FeSI and FeSII clusters Pseudomonas putida
Iron-sulfur-center
-
Pseudomonas putida
Molybdenum contains molybdenum-molybdopterin Pseudomonas putida
molybdenum-center
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46238
-
2 * 91041 + 2 * 46238, MALDI-TOF mass spectrometry Pseudomonas putida
51000
-
2 * 94000 + 2 * 51000, SDS-PAGE Pseudomonas putida
91041
-
2 * 91041 + 2 * 46238, MALDI-TOF mass spectrometry Pseudomonas putida
94000
-
2 * 94000 + 2 * 51000, SDS-PAGE Pseudomonas putida
580000
-
SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxanthine + NAD+ + H2O Pseudomonas putida
-
xanthine + NADH + H+
-
?
xanthine + NAD+ + H2O Pseudomonas putida
-
urate + NADH
-
?
xanthine + O2 + H2O Pseudomonas putida 4% of the activity compared to xanthine-NAD+ urate + O2- + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida 86
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, DEAE-10 Macro-Prep MP10 column chromatography, and Bio-Prep SE-1000/17 column chromatography Pseudomonas putida

Source Tissue

Source Tissue Comment Organism Textmining
cell culture inducible by hypoxanthine Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.9
-
crude extract Pseudomonas putida
26.7
-
hypoxanthine-NAD+ assay Pseudomonas putida
35.2
-
after 39.1fold purification Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzaldehyde + NAD+ + H2O 1.2% activity compared to xanthine Pseudomonas putida 3,4-dihydroxybenzoate + NADH + H+
-
?
3,4-dihydroxybenzaldehyde + NAD+ + H2O 1.2% activity compared to xanthine Pseudomonas putida 86 3,4-dihydroxybenzoate + NADH + H+
-
?
4-dimethylaminobenzaldehyde + NAD+ + H2O 0.3% activity compared to xanthine Pseudomonas putida 4-dimethylaminobenzoate + NADH
-
?
4-hydroxybenzaldehyde + NAD+ + H2O 0.7% activity compared to xanthine Pseudomonas putida 4-hydroxybenzoate + NADH + H+
-
?
4-hydroxybenzaldehyde + NAD+ + H2O 0.7% activity compared to xanthine Pseudomonas putida 86 4-hydroxybenzoate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O 1.3% activity compared to xanthine Pseudomonas putida benzoate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O 1.3% activity compared to xanthine Pseudomonas putida 86 benzoate + NADH + H+
-
?
hypoxanthine + ferricyanide + H2O
-
Pseudomonas putida xanthine + ferrocyanide
-
?
hypoxanthine + ferricyanide + H2O
-
Pseudomonas putida 86 xanthine + ferrocyanide
-
?
hypoxanthine + NAD+ + H2O
-
Pseudomonas putida xanthine + NADH + H+
-
?
hypoxanthine + NAD+ + H2O 149% activity compared to xanthine Pseudomonas putida urate + ? + NADH
-
?
additional information NAD+ is the most effcient electron acceptor, followed by 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride and ferricyanide Pseudomonas putida ?
-
?
additional information NAD+ is the most effcient electron acceptor, followed by 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride and ferricyanide Pseudomonas putida 86 ?
-
?
phthalazine + NAD+ + H2O 0.4% activity compared to xanthine Pseudomonas putida 1-(2H)-phthalazinone + NADH
-
?
purine + NAD+ + H2O 5% activity compared to xanthine Pseudomonas putida 8-hydroxypurine + NADH + H+
-
?
quinazoline + NAD+ + H2O 2% activity compared to xanthine Pseudomonas putida 4-(3H)-quinazolinone + NADH
-
?
xanthine + ferricyanide + H2O 58% of the activity compared to xanthine-NAD+ Pseudomonas putida urate + ferrocyanide
-
?
xanthine + NAD+ + H2O
-
Pseudomonas putida urate + NADH
-
?
xanthine + NAD+ + H2O 100% activity Pseudomonas putida urate + NADH
-
?
xanthine + O2 + H2O 4% of the activity compared to xanthine-NAD+ Pseudomonas putida urate + O2- + 2 H+
-
?

Subunits

Subunits Comment Organism
heterooctamer 2 * 91041 + 2 * 46238, MALDI-TOF mass spectrometry Pseudomonas putida
heterooctamer 2 * 94000 + 2 * 51000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
XDH
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas putida
additional information cofactor compostion similar to eukaryotic enzymes Pseudomonas putida
NAD+
-
Pseudomonas putida
NADP+
-
Pseudomonas putida