Literature summary for 1.17.1.4 extracted from

Adams, B.; Lowe, D.J.; Smith, A.T.; Scazzocchio, C.; Demais, S.; Bray, R.C.
Expression of Drosophila melanogaster xanthine dehydrogenase in Aspergillus nidulans and some properties of the recombinant enzyme (2002), Biochem. J., 362, 223-229.

Search for more literature for 1.17.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Aspergillus nidulans	Drosophila melanogaster

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.032	-	xanthine	electron acceptor: NAD+	Drosophila melanogaster

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron-sulfur-center	-	Drosophila melanogaster
molybdenum-center	-	Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
xanthine + NAD+ + H2O	Drosophila melanogaster	-	urate + NADH	-	?

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity, recombinant enzyme	Drosophila melanogaster

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetaldehyde + NAD+ + H2O	considerable activity for the recombinant enzyme	Drosophila melanogaster	acetate + NADH + H+	-	?
xanthine + NAD+ + H2O	-	Drosophila melanogaster	urate + NADH	-	?
xanthine + phenazine methosulfate + cytochrome c + H2O	-	Drosophila melanogaster	urate + ?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
31	-	xanthine	recombinant enzyme, electron acceptor: phenazine methosulfate-cytochrome c	Drosophila melanogaster

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
9.2	-	recombinant enzyme, half-life: 10-15 h at 0-4°C	Drosophila melanogaster

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Drosophila melanogaster
additional information	-	Drosophila melanogaster
NAD+	-	Drosophila melanogaster

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Release 2023.1 (January 2023)