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Literature summary for 1.16.1.1 extracted from
- Mercuric reductase activity of multiple heavy metal-resistant Lysinibacillus sphaericus G1 (2015), J. Basic Microbiol., 55, 285-992.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene merA, DNA and amino acid sequence determination and analysis, pylogenetic analysis and tree, recombinant expression in Escherichia coli strain BL21(DE3) | Lysinibacillus sphaericus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hg + NADP+ + H+ | Lysinibacillus sphaericus | - |
Hg2+ + NADPH | - |
r |  |
| Hg + NADP+ + H+ | Lysinibacillus sphaericus G1 | - |
Hg2+ + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lysinibacillus sphaericus | D9J041 | i.e. Bacillus sphaericus, isolated from an industrial mercuric salt-contaminated soil, gene merA | - |
| Lysinibacillus sphaericus G1 | D9J041 | i.e. Bacillus sphaericus, isolated from an industrial mercuric salt-contaminated soil, gene merA | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | growth of strain G1 at sublethal concentrations of metal salts, Cd2+, Zn2+, Co2+, and K2Cr2O7-, in presence of absence of Hg2+, overview | Lysinibacillus sphaericus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.13 | - |
cell extract, mercury-dependent oxidation of NADPH, pH 7.4, 25°C | Lysinibacillus sphaericus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hg + NADP+ + H+ | - |
Lysinibacillus sphaericus | Hg2+ + NADPH | - |
r | |
| Hg + NADP+ + H+ | - |
Lysinibacillus sphaericus G1 | Hg2+ + NADPH | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MerA | - |
Lysinibacillus sphaericus |
| mercuric reductase | - |
Lysinibacillus sphaericus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Lysinibacillus sphaericus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Lysinibacillus sphaericus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Lysinibacillus sphaericus | |
| NADPH | - |
Lysinibacillus sphaericus | |
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Release 2023.1 (January 2023)
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