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Literature summary for 1.16.1.1 extracted from
- Activation of mercuric reductase by the substrate NADPH (1987), Eur. J. Biochem., 164, 243-249.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | 1 mM, 30 min preincubation, relative initial rate 121% | Pseudomonas aeruginosa |  |
| cysteine | 30 mM, 30 min preincubation, relative initial rate 200% | Pseudomonas aeruginosa | |
| EDTA | no effect | Pseudomonas aeruginosa | |
| EDTA | 1 mM, 30 min preincubation, relative initial rate 104% | Pseudomonas aeruginosa | |
| NADP+ | 100 microM, 30 min preincubation, relative initial rate 108% | Pseudomonas aeruginosa | |
| NADPH | 100 microM, 30 min preincubation, relative initial rate 151%, 10 min preincubation with NADPH results in an increase of reactive thiol groups | Pseudomonas aeruginosa | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effective product inhibition by NADP+ | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
NADPH | in presence of 1 mM cysteine | Pseudomonas aeruginosa | |
| 0.0004 | - |
NADPH | 25°C, pH 7.3 | Pseudomonas aeruginosa | |
| 0.0032 | - |
Hg2+ | in presence of 1 mM cysteine | Pseudomonas aeruginosa | |
| 0.0032 | - |
Hg2+ | 1 mM cysteine, 25°C, pH 7.3 | Pseudomonas aeruginosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas aeruginosa | - |
Tn501-encoded | - |
| Pseudomonas aeruginosa PAO 9501 | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| activated enzyme appears to be stable under anaerobic conditions and eventually returns to the original level of activity in the presence of oxygen. The activated state seems to be stabilized by 1mM cysteine. | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hg2+ + NADPH | - |
Pseudomonas aeruginosa | Hg + NADP+ + H+ | - |
? | |
| Hg2+ + NADPH | - |
Pseudomonas aeruginosa PAO 9501 | Hg + NADP+ + H+ | - |
? | |
| NADPH + Hg2+ | - |
Pseudomonas aeruginosa | NADP+ + Hg | - |
? | |
| NADPH + Hg2+ | - |
Pseudomonas aeruginosa PAO 9501 | NADP+ + Hg | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mercuric reductase | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudomonas aeruginosa | |
| NADH | some activation | Pseudomonas aeruginosa | |
| NADPH | - |
Pseudomonas aeruginosa | |
| NADPH | in presence of 1 mM cysteine only one equivalent of NADPH per FAD is required for full activation | Pseudomonas aeruginosa | |
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