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Literature summary for 1.15.1.1 extracted from
- Superoxide dismutase (SOD) in boar spermatozoa: purification, biochemical properties and changes in activity during semen storage (16?C) in different extenders (2013), Reprod. Biol., 13, 34-40.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 15% inhibition | Sus scrofa |  |
| diethyldithiocarbamate | 40% inhibition | Sus scrofa | |
| H2O2 | 65% inhibition | Sus scrofa | |
| additional information | urea and iodoacetamide do not affect the enzymatic activity | Sus scrofa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, SDS-PAGE | Sus scrofa |
| 69000 | - |
- |
Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
Large White boars | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from sperm 7.5-38fold, superoxide dismutase release from spermatozoa after cold shock and homogenization, followed by ion exchange chromatography and gel filtration | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| sperm | changes in SOD activity in boar sperm during preservation at 16°C, overview | Sus scrofa | - |
| spermatozoon | - |
Sus scrofa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 16°C, changes in superoxide dismutase activity in boar sperm during preservation, overview | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | superoxide dismutase activity is measured by the inhibition of nitro blue tetrazolium reduction in the presence of the superoxide anion generated by the xanthine and xanthine oxidase system | Sus scrofa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 30000, SDS-PAGE | Sus scrofa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SOD | - |
Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
monomeric enzyme | Sus scrofa |
| 20 | 45 | dimeric enzyme, broad optimum | Sus scrofa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | high activity within this range | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Sus scrofa |
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