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Literature summary for 1.15.1.1 extracted from

  • Mandelli, F.; Franco Cairo, J.P.; Citadini, A.P.; Buechli, F.; Alvarez, T.M.; Oliveira, R.J.; Leite, V.B.; Paes Leme, A.F.; Mercadante, A.Z.; Squina, F.M.
    The characterization of a thermostable and cambialistic superoxide dismutase from Thermus filiformis (2013), Lett. Appl. Microbiol., 57, 40-46.
    View publication on PubMed
Search for more literature for 1.15.1.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison, expression of soluble enzyme in Escherichia coli strain BL21(DE3) Thermus filiformis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a cambialistic Mn/Fe-SOD Thermus filiformis
Mn2+ a cambialistic Mn/Fe-SOD Thermus filiformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, SDS-PAGE Thermus filiformis

Organism

Organism UniProt Comment Textmining
Thermus filiformis M9QJD2
-
-
Thermus filiformis ATCC 43280 M9QJD2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Thermus filiformis

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Thermus filiformis

Synonyms

Synonyms Comment Organism
SOD
-
 Thermus filiformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Thermus filiformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 purified recombinant enzyme, inactivation Thermus filiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8.2 assay at Thermus filiformis

General Information

General Information Comment Organism
additional information the amount of enzyme required to inhibit 50% of pyrogallol autoxidation is 0.41, 0.56 and 13.73 mg at 65°C, 70°C, and 80°C, respectively Thermus filiformis