Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.15.1.1 extracted from

  • Areekit, S.; Kanjanavas, P.; Khawsak, P.; Pakpitchareon, A.; Potivejkul, K.; Chansiri, G.; Chansiri, K.
    Cloning, expression, and characterization of thermotolerant manganese superoxide dismutase from Bacillus sp. MHS47 (2011), Int. J. Mol. Sci., 12, 844-856.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.15.1.1

Application

Application Comment Organism
diagnostics the enzyme might be used to develop a biosensor for the detection of antioxidants and free radical activity Bacillus sp. (in: Bacteria)

Cloned(Commentary)

Cloned (Comment) Organism
gene sodA, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by hydrogen peroxide or potassium cyanide Bacillus sp. (in: Bacteria)
Sodium azide
-
 Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ a Mn-superoxide dismutase, conserved manganese-binding site residues are H28, H83, D165, and H169 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22650
-
 x * 22650, sequence calculation Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 O2.- + 2 H+ Bacillus sp. (in: Bacteria)
-
 O2 + H2O2
-
 ?
2 O2.- + 2 H+ Bacillus sp. (in: Bacteria) MHS47
-
 O2 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) E9LK91 collected from soil and water samples from hot springs in Mae Hong Son Province of Thailand, gene sodA
-
Bacillus sp. (in: Bacteria) MHS47 E9LK91 collected from soil and water samples from hot springs in Mae Hong Son Province of Thailand, gene sodA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 14fold from Escherichia coli strain strain BL21(DE3) by nickel affinity chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3538
-
 pH 7.5, 37°C, recombinant enzyme Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 O2.- + 2 H+
-
 Bacillus sp. (in: Bacteria) O2 + H2O2
-
 ?
2 O2.- + 2 H+
-
 Bacillus sp. (in: Bacteria) MHS47 O2 + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 22650, sequence calculation Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
MnSOD47
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 80 activity range, profile overview Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 purified recombinant His-tagged enzyme, 40 min, 50% activity remaining Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
5 10 activity range, profile overview Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria) sequence calculation
-
 5.31

General Information

General Information Comment Organism
additional information MnSOD47 contains the decapod crustacean signature (DXWEHXXY), which is a specific characteristic of Mn-superoxide dismutase Bacillus sp. (in: Bacteria)
physiological function superoxide dismutases are considered key enzymes in the control of oxidative stress because they can protect oxygen-metabolizing cells against the harmful effects of superoxide free radicals Bacillus sp. (in: Bacteria)