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Literature summary for 1.15.1.1 extracted from

  • Haikarainen, T.; Frioux, C.; Zhnag, L.Q.; Li, D.C.; Papageorgiou, A.C.
    Crystal structure and biochemical characterization of a manganese superoxide dismutase from Chaetomium thermophilum (2014), Biochim. Biophys. Acta, 1844, 422-429.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mnsod, expression in Pichia pastoris strain GS115. Transformed recombinant yeast cells exhibit higher stress resistance to salt and oxidative stress-inducing agents than control yeast cells Thermochaetoides thermophila

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant Mn-SOD enzyme, X-ray diffraction structure determination and analysis at 2.0 A resolution Thermochaetoides thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ manganese superoxide dismutase Thermochaetoides thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
x * 23000, SDS-PAGE Thermochaetoides thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 O2.- + 2 H+ Thermochaetoides thermophila
-
O2 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila F8V325
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Pichia pastoris strain GS115 by anion exchange chromatography Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 O2.- + 2 H+
-
Thermochaetoides thermophila O2 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 23000, SDS-PAGE Thermochaetoides thermophila
More subunit interface structure, overview Thermochaetoides thermophila

Synonyms

Synonyms Comment Organism
manganese superoxide dismutase
-
Thermochaetoides thermophila
MnSOD
-
Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
an increased number of charged residues and an increase in the number of intersubunit salt bridges and the Thr:Ser ratio compared to enzymes from other species are identified as potential reasons for the thermostability of CtMnSOD Thermochaetoides thermophila
50 60 purified recombinant enzyme, 60 min, stable Thermochaetoides thermophila
70
-
purified recombinant enzyme, 60 min, 75% activity remaining Thermochaetoides thermophila
80
-
purified recombinant enzyme, 25 min, 50% activity remaining Thermochaetoides thermophila
90
-
purified recombinant enzyme, 30 min, 20% activity remaining Thermochaetoides thermophila

General Information

General Information Comment Organism
additional information structure analysis by molecular replacement using human mitochondrial MnSOD variant, PDB ID 1var, modelling Thermochaetoides thermophila