Cloned (Comment) | Organism |
---|---|
gene mnsod, expression in Pichia pastoris strain GS115. Transformed recombinant yeast cells exhibit higher stress resistance to salt and oxidative stress-inducing agents than control yeast cells | Thermochaetoides thermophila |
Crystallization (Comment) | Organism |
---|---|
purified recombinant Mn-SOD enzyme, X-ray diffraction structure determination and analysis at 2.0 A resolution | Thermochaetoides thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | manganese superoxide dismutase | Thermochaetoides thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 23000, SDS-PAGE | Thermochaetoides thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 O2.- + 2 H+ | Thermochaetoides thermophila | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | F8V325 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Pichia pastoris strain GS115 by anion exchange chromatography | Thermochaetoides thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 O2.- + 2 H+ | - |
Thermochaetoides thermophila | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE | Thermochaetoides thermophila |
More | subunit interface structure, overview | Thermochaetoides thermophila |
Synonyms | Comment | Organism |
---|---|---|
manganese superoxide dismutase | - |
Thermochaetoides thermophila |
MnSOD | - |
Thermochaetoides thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
an increased number of charged residues and an increase in the number of intersubunit salt bridges and the Thr:Ser ratio compared to enzymes from other species are identified as potential reasons for the thermostability of CtMnSOD | Thermochaetoides thermophila |
50 | 60 | purified recombinant enzyme, 60 min, stable | Thermochaetoides thermophila |
70 | - |
purified recombinant enzyme, 60 min, 75% activity remaining | Thermochaetoides thermophila |
80 | - |
purified recombinant enzyme, 25 min, 50% activity remaining | Thermochaetoides thermophila |
90 | - |
purified recombinant enzyme, 30 min, 20% activity remaining | Thermochaetoides thermophila |
General Information | Comment | Organism |
---|---|---|
additional information | structure analysis by molecular replacement using human mitochondrial MnSOD variant, PDB ID 1var, modelling | Thermochaetoides thermophila |