Literature summary for 1.15.1.1 extracted from

Gazdag, E.M.; Cirstea, I.C.; Breitling, R.; Lukes, J.; Blankenfeldt, W.; Alexandrov, K.
Purification and crystallization of human Cu/Zn superoxide dismutase recombinantly produced in the protozoan Leishmania tarentolae (2010), Acta Crystallogr. Sect. F, 66, 871-877.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of SOD1 in Leishmania tarentolae strain P10	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant SOD1, hanging drop vapour diffusion, 0.001 ml of 10 mg/ml protein in 50 mM sodium citrate, pH 5.5, 1 mM DTT, 100 mM CuSO4, and 100 mM ZnSO4, is mixed with 0.001 ml of reservoir solution containing 21-25% w/v PEG 4000, 0.1 M sodium acetate, pH 4.2-5.2, X-ray diffraction structure determination and analysis at 3.5 A, molecular replacement	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	a CuZn-superoxide dismutase	Homo sapiens
Zn2+	a CuZn-superoxide dismutase	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P00441	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant SOD1 from Leishmania tarentolae strain P10 to 90% purity by ultracentrifugation, hydrophobic interaction chromatography and dialysis	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Cu/Zn superoxide dismutase	-	Homo sapiens
SOD1	-	Homo sapiens

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Release 2023.1 (January 2023)