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Literature summary for 1.15.1.1 extracted from

  • Guo, F.X.; Shi-Jin, E.; Liu, S.A.; Chen, J.; Li, D.C.
    Purification and characterization of a thermostable MnSOD from the thermophilic fungus Chaetomium thermophilum (2008), Mycologia, 100, 375-380.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by KCN and H2O2 Thermochaetoides thermophila
NaN3
-
Thermochaetoides thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.00205 mg/mg of protein Thermochaetoides thermophila
additional information the purified enzyme contains no Fe Thermochaetoides thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23500
-
4 * 23500, SDS-PAGE Thermochaetoides thermophila
94400
-
gel filtration Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila
-
-
-
Thermochaetoides thermophila CT2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 7.49fold to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and hydrophobic interaction chromatography Thermochaetoides thermophila

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
115.8
-
purified native enzyme Thermochaetoides thermophila

Subunits

Subunits Comment Organism
More the enzyme contains the sequence TLPDLKYD at the N-terminus Thermochaetoides thermophila
tetramer 4 * 23500, SDS-PAGE Thermochaetoides thermophila

Synonyms

Synonyms Comment Organism
MnSOD
-
Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermochaetoides thermophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 90
-
Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 purified enzyme, completely stable Thermochaetoides thermophila
70
-
60 min, 60% activity remaining Thermochaetoides thermophila
80
-
half-life of the purified enzyme is about 25 min Thermochaetoides thermophila
90
-
purified enzyme, 30 min, 20% activity remaining Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Thermochaetoides thermophila

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
Thermochaetoides thermophila