Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, real-time RT-PCR expression analysis, sequence comparisons, expression in Escherichia coli strain QC779 | Danio rerio |
General Stability | Organism |
---|---|
the enzyme is stable in presence of 4% SDS | Danio rerio |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn | a MnSOD | Danio rerio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24989 | - |
x * 24989, sequence calculation, MnSOD | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 O2.- + 2 H+ | Danio rerio | - |
O2 + H2O2 | - |
? | |
2 O2.- + 2 H+ | Danio rerio AB | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q2TV65 | - |
- |
Danio rerio AB | Q2TV65 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain QC779 | Danio rerio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | MnSOD developmental expression, overview | Danio rerio | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 O2.- + 2 H+ | - |
Danio rerio | O2 + H2O2 | - |
? | |
2 O2.- + 2 H+ | - |
Danio rerio AB | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24989, sequence calculation, MnSOD | Danio rerio |
More | MnSOD molecular modelling of the highly conserved sequence, structure modelling and comparison with MnSODs from other organisms | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
manganese superoxide dismutase | - |
Danio rerio |
Mn-SOD | - |
Danio rerio |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal inactivation kinetics fit the first-order inactivation rate equation, recombinant enzyme | Danio rerio |
70 | - |
purified recombinant enzyme, half-life is 48 min | Danio rerio |
80 | - |
purified recombinant enzyme, 48% remaining activity after 10 min | Danio rerio |
90 | - |
purified recombinant enzyme, inactivation after 10 min | Danio rerio |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.2 | 11.2 | high enzyme activity | Danio rerio |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Danio rerio | sequence calculation, MnSOD | - |
8.29 |