Cloned (Comment) | Organism |
---|---|
His-tagged MnSOD-2 and MnSOD-3 | Caenorhabditis elegans |
Crystallization (Comment) | Organism |
---|---|
MnSOD-2 and MnSOD-3, at 3 and 8 mg/ml respectively, in 10 mM Tris-HCl, pH 7.8, mixing of 0.001 ml protein and reservoir solution, the latter containing 0.1 M bicine pH 9.2 and 3.0 M ammonium sulfate for MnSOD-2, and 0.1 M bicine, pH 9.2, and 2.7 M ammonium sulfate for MnSOD-3, X-ray diffraction structure determination and analysis at 1.7-1.8 A resolution | Caenorhabditis elegans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | MnSOD-2 and MnSOD-3 | Caenorhabditis elegans | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn | Mn-SOD | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged MnSOD-2 and MnSOD-3 by nickel affinity and anion exchange chromatography, followed by gel filtration | Caenorhabditis elegans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | MnSOD-2 is constitutively expressed, while synthesis of MnSOD-3 is inducible | Caenorhabditis elegans | - |
Synonyms | Comment | Organism |
---|---|---|
manganese superoxide dismutase | - |
Caenorhabditis elegans |
MnSOD-2 | - |
Caenorhabditis elegans |
MnSOD-3 | - |
Caenorhabditis elegans |