Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a Cu,Zn-SOD | Aspergillus niger | |
Mn2+ | a Mn-SOD | Aspergillus niger | |
Zn2+ | a Cu,Zn-SOD | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15821 | - |
2 * 15821, Cu,Zn-SOD, sequence calculation | Aspergillus niger |
22340 | - |
4 * 22340, Mn-SOD, sequence calculation | Aspergillus niger |
30000 | - |
Cu,Zn-SOD, native PAGE | Aspergillus niger |
88000 | - |
Mn-SOD, native PAGE | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2.- + H+ | Aspergillus niger | - |
O2 + H2O2 | - |
? | |
O2.- + H+ | Aspergillus niger 26 | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger 26 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native Cu,Zn-SOD about 13fold by two steps of hydrophobic interaction chromatography and two steps of anion exchange chromatography | Aspergillus niger |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 superoxide + 2 H+ = O2 + H2O2 | the amino acid residues His46, His48, His63, His71, His80, and His120, and Asp83 in the active site are conserved as in other Cu/ZnSODs | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2940 | - |
purified Cu,Zn-SOD | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | SOD activity measurement using the nitroblue tetrazolium | Aspergillus niger | ? | - |
? | |
additional information | SOD activity measurement using the nitroblue tetrazolium | Aspergillus niger 26 | ? | - |
? | |
O2.- + H+ | - |
Aspergillus niger | O2 + H2O2 | - |
? | |
O2.- + H+ | - |
Aspergillus niger 26 | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 15821, Cu,Zn-SOD, sequence calculation | Aspergillus niger |
More | peptide mapping by tryptic digest and amino acid sequence determination and analysis, the enzyme does neither contain a Tyr residue nor a carbohydrate chain occupying an N-linkage site -N-I-Y-,overview, homology modeling of Cu/Zn-SOD | Aspergillus niger |
tetramer | 4 * 22340, Mn-SOD, sequence calculation | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
Cu/Zn-SOD | - |
Aspergillus niger |
Cu/Zn-superoxide dismutase | - |
Aspergillus niger |
Mn-SOD | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 85 | at pH 7.8, temperature profile, overview | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, 53% remaining activity | Aspergillus niger |
85 | - |
30 min, 15% remaining activity | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 12 | 90% of maximal activity at pH 5.6-pH 7.5, 15% at pH 4.0, 60% at pH 12.0, 25°C, pH profile, overview | Aspergillus niger |