Literature summary for 1.15.1.1 extracted from

Rodriguez-Serrano, M.; Romero-Puertas, M.C.; Pastori, G.M.; Corpas, F.J.; Sandalio, L.M.; del Rio, L.A.; Palma, J.M.
Peroxisomal membrane manganese superoxide dismutase: characterization of the isozyme from watermelon (Citrullus lanatus Schrad.) cotyledons (2007), J. Exp. Bot., 58, 2417-2427.

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	mitMn-SOD	Citrullus lanatus	5739	-
peroxisomal membrane	bound, perMn-SOD	Citrullus lanatus	5778	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	4 * 27000, peroxisomal Mn-SOD, SDS-PAGE	Citrullus lanatus
108000	-	peroxisomal Mn-SOD, gel filtration	Citrullus lanatus
112000	-	mitochondrial Mn-SOD, gel filtration	Citrullus lanatus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
O2.- + H+	Citrullus lanatus	-	O2 + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Citrullus lanatus	Q6QGY4	cv. sugar baby	-

Purification (Commentary)

Purification (Comment)	Organism
native peroxisomal Mn-SOD 5600fold from peroxisomal membranes, to homogeneity by ammonium sulfate fractionation, batch anion-exchange chromatography, and anion-exchange and gel filtration, mitochondrial Mn-SOD partially	Citrullus lanatus

Source Tissue

Source Tissue	Comment	Organism	Textmining
cotyledon	-	Citrullus lanatus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
480	-	purified Mn-SOD from peroxisomal membrane	Citrullus lanatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	SOD activity measurement by the ferricytochrome c method, using xanthine/xanthine oxidase as the source of superoxide radicals	Citrullus lanatus	?	-	?
O2.- + H+	-	Citrullus lanatus	O2 + H2O2	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 27000, peroxisomal Mn-SOD, SDS-PAGE	Citrullus lanatus

Synonyms

Synonyms	Comment	Organism
manganese superoxide dismutase	-	Citrullus lanatus
mitMn-SOD	-	Citrullus lanatus
perMn-SOD	-	Citrullus lanatus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Citrullus lanatus	mitochondrial Mn-SOD, isoelectric focusing	-	5.66
Citrullus lanatus	peroxisomal Mn-SOD, isoelectric focusing	-	5.75

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Release 2023.1 (January 2023)