Cloned (Comment) | Organism |
---|---|
gene sod, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109(DE3) | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenylmethanesulfonyl fluoride | irreversible inactivation by attachment of a molecule phenylmethanesulfonyl fluoride to the active site Tyr41 reinforcing the heat stability of the enzyme, overview | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe-type SOD | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
4 * 24000, recombinant enzyme, SDS-PAGE | Saccharolobus solfataricus |
88000 | - |
recombinant enzyme, gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P80857 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme, and recombinant enzyme from Escherichia coli strain JM109(DE3) | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | native and recombinant enzyme ossess a covalent modification of the conserved Tyr41 in the active site, Tyr41 plays an important role in the enzyme activity and the maintenance of the structural architecture of SOD, overview | Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 24000, recombinant enzyme, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
SOD | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
phenylmethanesulfonyl fluoride attachment to the active site Tyr41 increases the heat stability of the enzyme, overview | Saccharolobus solfataricus |