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Literature summary for 1.15.1.1 extracted from

  • Gogliettino, M.A.; Tanfani, F.; Scire, A.; Ursby, T.; Adinolfi, B.S.; Cacciamani, T.; De Vendittis, E.
    The role of Tyr41 and His155 in the functional properties of superoxide dismutase from the archaeon Sulfolobus solfataricus (2004), Biochemistry, 43, 2199-2208.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain JM109(DE3) Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified mutant enzyme Y41F, hanging drop vapor diffusion method, 21°C, 1:1 mix of the reservoir solution containing 8% PEG 8000, 0.1 M Tris-HCl, pH 8.5, and the protein solution containing 1.45 mg/mL Y41F, 20 mM Tris-HCl, pH 7.8, and 1% glycerol, X-ray diffraction structure determination and analysis Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
H155Q site-directed mutagenesis, the mutant shows a a slightly lower iron content, reduced heat stability, and a 2fold reduced activity compared to the wild-type enzyme Saccharolobus solfataricus
Y41F site-directed mutagenesis, the mutant shows a a slightly lower iron content and a 17fold reduced activity compared to the wild-type enzyme, the mutant shows an uninterrupted hydrogen bond network Saccharolobus solfataricus

General Stability

General Stability Organism
SDS decreases the stability of SOD and accelerates enzyme inactivation Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
NaN3 inhibits the enzyme mutants Y41F and H155Q, but not the wild-type enzyme Saccharolobus solfataricus
PMSF irreversible inhibition by binding to active site Tyr41 Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe-type SOD Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
4 * 24000, wild-type and mutant enzymes, SDS-PAGE Saccharolobus solfataricus
87900
-
wild-type and mutant enzymes, gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
387
-
purified mutant Y41F Saccharolobus solfataricus
3980
-
purified mutant H155Q Saccharolobus solfataricus
6720
-
purified wild-type enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information covalent modification of the conserved Tyr41 in the active site, Tyr41 and His155 are involved in catalysis, hydrogen bond network including three solvent molecules connecting the iron-ligating hydroxide ion via H155 with F41 and H37, Y41 and H155 are important for the structural and functional properties of SOD, overview Saccharolobus solfataricus ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 24000, wild-type and mutant enzymes, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SOD
-
Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7.4 assay at Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus isoelectric focusing
-
8.4