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Literature summary for 1.15.1.1 extracted from

  • Lumsden, J.; Cammack, R.; Hall, D.O.
    Purification and physicochemical properties of superoxide dismutase from two photosynthetic microorganisms (1976), Biochim. Biophys. Acta, 438, 380-392.
    View publication on PubMed

General Stability

General Stability Organism
dimethyl sulfoxide: Spirulina enzyme is stable up to 55%, Rhodopseudomonas enzyme up to 70% v/v Cereibacter sphaeroides
dimethyl sulfoxide: Spirulina enzyme is stable up to 55%, Rhodopseudomonas enzyme up to 70% v/v Arthrospira platensis

Inhibitors

Inhibitors Comment Organism Structure
H2O2 Fe-SOD Arthrospira platensis
H2O2 no inhibition: Mn-SOD Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 1.0-1.45 mol per mol of enzyme Arthrospira platensis
Mn2+ 1 atom per subunit Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18100
-
2 * 18100, Fe-SOD, SDS-PAGE Arthrospira platensis
18300
-
2 * 18300, Mn-SOD, SDS-PAGE Cereibacter sphaeroides
37400
-
gel filtration Cereibacter sphaeroides
37400
-
gel filtration Arthrospira platensis
37400
-
Fe-SOD Arthrospira platensis
37400
-
Mn-SOD Cereibacter sphaeroides

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl sulfoxide stable up to 55% v/v Arthrospira platensis
dimethyl sulfoxide stable up to 70% v/v Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Arthrospira platensis
-
Fe-SOD
-
Arthrospira platensis Fe-SOD
-
Fe-SOD
-
Cereibacter sphaeroides
-
aerobically grown
-
Cereibacter sphaeroides
-
Mn-SOD
-
Cereibacter sphaeroides Mn-SOD
-
Mn-SOD
-

Purification (Commentary)

Purification (Comment) Organism
Fe-SOD Arthrospira platensis
Mn-SOD Cereibacter sphaeroides

Reaction

Reaction Comment Organism Reaction ID
2 superoxide + 2 H+ = O2 + H2O2 amino acid composition, comparison Cereibacter sphaeroides
2 superoxide + 2 H+ = O2 + H2O2 amino acid composition, comparison Arthrospira platensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O2- + H+
-
Cereibacter sphaeroides O2 + H2O2
-
?
O2- + H+
-
Arthrospira platensis O2 + H2O2
-
?
O2- + H+
-
Arthrospira platensis Fe-SOD O2 + H2O2
-
?
O2- + H+
-
Cereibacter sphaeroides Mn-SOD O2 + H2O2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 18300, Mn-SOD, SDS-PAGE Cereibacter sphaeroides
dimer 2 * 18100, Fe-SOD, SDS-PAGE Arthrospira platensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
half-life: 6.5 min Arthrospira platensis
70
-
half-life: 12.75 min Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
Mn-SOD Cereibacter sphaeroides
7.8
-
Fe-SOD Arthrospira platensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10.8 30 min, pH 3.0: about 30% loss of activity, pH 10.8: rapid inactivation above Cereibacter sphaeroides
5 10.8 30 min, unstable below pH 5.5 and above pH 10.8 Arthrospira platensis