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Literature summary for 1.15.1.1 extracted from
- Purification and characterization of an iron-containing superoxide dismutase from a eucaryote, Ginkgo biloba (1985), Arch. Biochem. Biophys., 243, 305-314.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CN- | no inhibition | Ginkgo biloba |  |
| H2O2 | - |
Ginkgo biloba | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | Fe-SOD, stroma | Ginkgo biloba | 9507 | - |
| mitochondrion | Cu,Zn-SOD | Ginkgo biloba | 5739 | - |
| mitochondrion | Mn-SOD | Ginkgo biloba | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 2 mol of Cu per mol of enzyme | Ginkgo biloba | |
| Iron | 1.0-1.45 mol per mol of enzyme | Ginkgo biloba | |
| additional information | Cu2+ is not necessarily required | Ginkgo biloba | |
| Zn2+ | 2 mol of Zn2+ per mol of enzyme | Ginkgo biloba | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
Fe-SOD, gel filtration | Ginkgo biloba |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | Fe-SOD | Ginkgo biloba |
| chloroform | Fe-SOD | Ginkgo biloba |
| chloroform | Cu,Zn-SOD | Ginkgo biloba |
| Ethanol | Fe-SOD, Cu,Zn-SOD | Ginkgo biloba |
| additional information | chloroform/ethanol is used during purification of Fe-SOD and for detection of Cu,Zn-SOD, acetone precipitation | Ginkgo biloba |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ginkgo biloba | - |
Fe-SOD: 2 isozymes, contains also Zn2+ and Cu2+ | - |
| Ginkgo biloba | - |
Mn-SOD, Cu,Zn-SOD | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Fe-SOD, 2 isozymes | Ginkgo biloba |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 superoxide + 2 H+ = O2 + H2O2 | amino acid composition, comparison | Ginkgo biloba |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Ginkgo biloba | - |
| seed | Cu,Zn-SOD | Ginkgo biloba | - |
| seedling | etiolated, Mn-SOD and Cu,Zn-SOD | Ginkgo biloba | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ginkgo biloba |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 4 days, 50% loss of activity | Ginkgo biloba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O2- + H+ | - |
Ginkgo biloba | O2 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Ginkgo biloba |
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