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Literature summary for 1.14.99.1 extracted from

  • Ramkissoon, A.; Wells, P.G.
    Human prostaglandin H synthase (hPHS)-1- and hPHS-2-dependent bioactivation, oxidative macromolecular damage, and cytotoxicity of dopamine, its precursor, and its metabolites (2011), Free Radic. Biol. Med., 50, 295-304.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
arachidonic acid exogenous, increases hPHS activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of isozymes PHS-1 and PHS-2 in CHO-K1 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
DUP-697 a standard PHS-2 inhibitor Homo sapiens
SC-560 a standard PHS-1 inhibitor Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arachidonate + AH2 + O2 Homo sapiens
-
prostaglandin H2 + A + H2O
-
?
additional information Homo sapiens dopamine precursor L-dihydroxyphenylalanine, i.e. L-DOPA, and metabolites dihydroxyphenylacetic acid, homovanillic acid, and 3-methoxytyramine may serve as substrates for prostaglandin H synthase-catalyzed bioactivation to free radical intermediates ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + AH2 + O2
-
Homo sapiens prostaglandin H2 + A + H2O
-
?
additional information dopamine precursor L-dihydroxyphenylalanine, i.e. L-DOPA, and metabolites dihydroxyphenylacetic acid, homovanillic acid, and 3-methoxytyramine may serve as substrates for prostaglandin H synthase-catalyzed bioactivation to free radical intermediates Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
PHS-1
-
Homo sapiens
PHS-2
-
Homo sapiens
prostaglandin H synthase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

General Information

General Information Comment Organism
physiological function PHS isozyme-dependent oxidative damage to proteins and DNA, and cytotoxicity, overview. hPHS-1- and hPHS-2-expressing cells incubated with dopamine, L-dihydroxyphenylalanine, dihydroxyphenylacetic acid, or homovanillic acid exhibit increased cytotoxicity compared to untransfected cells, and cytotoxicity is increased further by exogenous arachidonic acid, which increases hPHS activity. Isozyme-specific, PHS-dependent oxidative damage and cytotoxicity caused by neurotransmitters, their precursors, and their metabolites may contribute to neurodegeneration associated with aging Homo sapiens