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Literature summary for 1.14.99.1 extracted from
- Role of Tyr348 in Tyr385 radical dynamics and cyclooxygenase inhibitor interactions in prostaglandin H synthase-2 (2006), Biochemistry, 45, 523-532.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pVL1393 vector | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y348F | mutant | Homo sapiens |
| Y348F/Y504F | mutant | Homo sapiens |
| Y504F | mutant | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aspirin | - |
Homo sapiens |  |
| nimesulide | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0021 | - |
arachidonic acid | wild-type, cyclooxygenase activity | Homo sapiens | |
| 0.0034 | - |
arachidonic acid | mutant Y348F/Y504F, cyclooxygenase activity | Homo sapiens | |
| 0.0038 | - |
arachidonic acid | mutant Y348F, cyclooxygenase activity | Homo sapiens | |
| 0.0042 | - |
arachidonic acid | mutant Y504F, cyclooxygenase activity | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
determined by SDS-PAGE and immunoblotting | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidonate + AH2 + O2 | Homo sapiens | - |
prostaglandin H2 + A + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0027 | - |
mutant Y348F, cyclooxygenase activity | Homo sapiens |
| 0.0039 | - |
mutant Y504F, cyclooxygenase activity | Homo sapiens |
| 0.0047 | - |
mutant Y348F/Y504F, cyclooxygenase activity | Homo sapiens |
| 0.0058 | - |
wild-type, cyclooxygenase activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidonate + AH2 + O2 | - |
Homo sapiens | prostaglandin H2 + A + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hPGHS-2 | - |
Homo sapiens |
| PGHS | - |
Homo sapiens |
| PGHS-2 | - |
Homo sapiens |
| prostaglandin H synthase | - |
Homo sapiens |
| prostaglandin H synthase-2 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
lipoxygenase activity assay at room temperature | Homo sapiens |
| 30 | - |
cyclooxygenase activity assay | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
lipoxygenase activity assay | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00027 | - |
nimesulide | mutant Y348F/Y504F, cyclooxygenase inhibition | Homo sapiens | |
| 0.0009 | - |
nimesulide | mutant Y504F, cyclooxygenase inhibition | Homo sapiens | |
| 0.001 | - |
nimesulide | wild-type, cyclooxygenase inhibition | Homo sapiens | |
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Release 2023.1 (January 2023)
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