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Literature summary for 1.14.19.2 extracted from
- Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis (2012), Planta, 236, 1665-1676.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Chromochloris zofingiensis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Chromochloris zofingiensis | 9507 | - |
| soluble | - |
Chromochloris zofingiensis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47040 | - |
x * 47040, calculated from amino acid sequence | Chromochloris zofingiensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chromochloris zofingiensis | D0V0B8 | - |
- |
| Chromochloris zofingiensis ATCC 30412 | D0V0B8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | - |
Chromochloris zofingiensis | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? |  |
| stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | - |
Chromochloris zofingiensis ATCC 30412 | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47040, calculated from amino acid sequence | Chromochloris zofingiensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SAD | - |
Chromochloris zofingiensis |
| stearoyl-ACP desaturase | - |
Chromochloris zofingiensis |
| stearoyl-acyl carrier protein desaturase | - |
Chromochloris zofingiensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | - |
Chromochloris zofingiensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Chromochloris zofingiensis | calculated from amino acid sequence | - |
6.4 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Chromochloris zofingiensis | exposure to HL (0.4 mM photons m-2 s-1) significantly increases the enzyme's mRNA level. Nitrogen deficiency also markedly up-regulates the expression of the enzyme. mRNA levels of the enzyme are drastically upregulated, reaching their maximum at 48 h after glucose induction | up |
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