Cloned (Comment) | Organism |
---|---|
OeSAD2 is not expressed as a soluble protein | Ophrys sphegodes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ophrys sphegodes | - |
- |
- |
Ophrys sphegodes subsp. sphegodes | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Ophrys sphegodes | - |
flower | - |
Ophrys sphegodes subsp. sphegodes | - |
leaf | - |
Ophrys sphegodes | - |
leaf | - |
Ophrys sphegodes subsp. sphegodes | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | in vitro analysis of the enzyme does not show any product | Ophrys sphegodes | ? | - |
? | |
additional information | lack of soluble OeSAD2 protein expression precludes its in vitro analysis | Ophrys sphegodes | ? | - |
? | |
additional information | OsSAD2 is active both on 18:0-[acyl-carrier protein] DELTA9 and 16:0-[acyl-carrier protein] DELTA4 substrates producing 18:1 DELTA9 and 16:1 DELTA4 products | Ophrys sphegodes | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OeSAD2 | - |
Ophrys sphegodes |
OeSAD3 | - |
Ophrys sphegodes subsp. sphegodes |
OsSAD1 | - |
Ophrys sphegodes |
OsSAD2 | - |
Ophrys sphegodes |
OsSAD3 | - |
Ophrys sphegodes |
stearoyl-acyl carrier protein desaturase | - |
Ophrys sphegodes |
stearoyl-acyl carrier protein desaturase | - |
Ophrys sphegodes subsp. sphegodes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Ophrys sphegodes |
General Information | Comment | Organism |
---|---|---|
malfunction | Ophrys SAD coding sequences are heterologously expressed in Arabidopsis under the control of the Cauliflower mosaic virus 35S RNA promoter. None of the transgenic plant lines complement the dwarf phenotype of homozygous ssi2 mutants. The presence of the OsSAD2 transgene is significantly associated with changes in unsaturated C18 and C16 FA levels in Arabidopsis leaf lipids, suggesting that OsSAD2 has enzymatic activity in Arabidopsis | Ophrys sphegodes |
malfunction | Ophrys SAD coding sequences are heterologously expressed in Arabidopsis under the control of the Cauliflower mosaic virus 35S RNA promoter. OeSAD2 does not complement the dwarf phenotype of homozygous ssi2 mutants | Ophrys sphegodes |
malfunction | Ophrys SAD coding sequences are heterologously expressed in Arabidopsis under the control of the Cauliflower mosaic virus 35S RNA promoter. OsSAD1 does not complement the dwarf phenotype of homozygous ssi2 mutants | Ophrys sphegodes |
physiological function | SAD2 is a florally expressed barrier gene of large phenotypic effect and, possibly, a genic target of pollinator-mediated selection | Ophrys sphegodes |