Cloned (Comment) | Organism |
---|---|
cloned in pQE-30 vector and expressed in Escherichia coli strain M15, His-tag | Jatropha curcas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
SDS-PAGE, overexpressed His-tag fusion protein | Jatropha curcas |
45510 | - |
precursor | Jatropha curcas |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
stearoyl-[acyl-carrier protein] + ferredoxin + O2 | Jatropha curcas | - |
oleoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Jatropha curcas | Q4JIJ4 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Jatropha curcas | - |
fruit | highly expressed in developing fruits | Jatropha curcas | - |
husk | young | Jatropha curcas | - |
leaf | young and mature | Jatropha curcas | - |
root | young | Jatropha curcas | - |
seed | young | Jatropha curcas | - |
stem | young | Jatropha curcas | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000003 | - |
His-tag fusion protein overexpressed in Escherichia coli M15 without ferredoxin, without IPTG-induction | Jatropha curcas |
0.000082 | - |
His-tag fusion protein overexpressed in Escherichia coli M15 without ferredoxin, IPTG-induced | Jatropha curcas |
0.00157 | - |
His-tag fusion protein overexpressed in Escherichia coli M15 with exogenous spinach ferredoxin, without IPTG-induction | Jatropha curcas |
0.00795 | - |
His-tag fusion protein overexpressed in Escherichia coli M15 with exogenous spinach ferredoxin, without IPTG-induced | Jatropha curcas |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
stearoyl-[acyl-carrier protein] + ferredoxin + O2 | - |
Jatropha curcas | oleoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | reduced spinach ferredoxin | Jatropha curcas |