Activating Compound | Comment | Organism | Structure |
---|---|---|---|
catalase | 3fold stimulation of purified enzyme, 4-5fold increase of activity in crude extracts | Carthamus tinctorius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00038 | - |
stearoyl-[acyl-carrier protein] | - |
Carthamus tinctorius | |
0.00051 | - |
palmitoyl-[acyl-carrier protein] | - |
Carthamus tinctorius | |
0.0083 | - |
stearoyl-CoA | - |
Carthamus tinctorius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, SDS-PAGE | Carthamus tinctorius |
68000 | - |
gel filtration | Carthamus tinctorius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
stearoyl-[acyl-carrier protein] + electron donor + O2 | Carthamus tinctorius | involved in oleic acid biosynthesis | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carthamus tinctorius | P22243 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Carthamus tinctorius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Carthamus tinctorius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
- |
Carthamus tinctorius |
Storage Stability | Organism |
---|---|
-70°C, 50% glycerol, 0.1% bovine serum albumin, 100 mM potassium phosphate buffer, pH 6.8, at least 2 months, no loss of activity | Carthamus tinctorius |
4°C, overnight, 30% loss of activity | Carthamus tinctorius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2 | 1% of activity with stearoyl-[acyl-carrier-protein] | Carthamus tinctorius | 9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
stearoyl-CoA + electron donor + O2 | 5% of activity with stearoyl-[acyl-carrier-protein] | Carthamus tinctorius | 9-octadecenoyl-CoA + acceptor + H2O | - |
? | |
stearoyl-[acyl-carrier protein] + electron donor + O2 | involved in oleic acid biosynthesis | Carthamus tinctorius | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? | |
stearoyl-[acyl-carrier protein] + reduced acceptor + O2 | enzyme is specific for stearoyl-CoA | Carthamus tinctorius | oleoyl-[acyl-carrier protein] + acceptor + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, SDS-PAGE | Carthamus tinctorius |