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Literature summary for 1.14.19.2 extracted from

  • McKeon, T.A.; Stumpf, P.K.
    Purification and characterization of the stearoyl-acyl carrier protein desaturase and the acyl-acyl carrier protein thioesterase from maturing seeds of safflower (1982), J. Biol. Chem., 257, 12141-12147.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
catalase 3fold stimulation of purified enzyme, 4-5fold increase of activity in crude extracts Carthamus tinctorius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00038
-
stearoyl-[acyl-carrier protein]
-
Carthamus tinctorius
0.00051
-
palmitoyl-[acyl-carrier protein]
-
Carthamus tinctorius
0.0083
-
stearoyl-CoA
-
Carthamus tinctorius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
2 * 36000, SDS-PAGE Carthamus tinctorius
68000
-
gel filtration Carthamus tinctorius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
stearoyl-[acyl-carrier protein] + electron donor + O2 Carthamus tinctorius involved in oleic acid biosynthesis oleoyl-[acyl-carrier protein] + acceptor + H2O
-
?

Organism

Organism UniProt Comment Textmining
Carthamus tinctorius P22243
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Carthamus tinctorius

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Carthamus tinctorius
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
-
Carthamus tinctorius

Storage Stability

Storage Stability Organism
-70°C, 50% glycerol, 0.1% bovine serum albumin, 100 mM potassium phosphate buffer, pH 6.8, at least 2 months, no loss of activity Carthamus tinctorius
4°C, overnight, 30% loss of activity Carthamus tinctorius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2 1% of activity with stearoyl-[acyl-carrier-protein] Carthamus tinctorius 9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
-
?
stearoyl-CoA + electron donor + O2 5% of activity with stearoyl-[acyl-carrier-protein] Carthamus tinctorius 9-octadecenoyl-CoA + acceptor + H2O
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2 involved in oleic acid biosynthesis Carthamus tinctorius oleoyl-[acyl-carrier protein] + acceptor + H2O
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2 enzyme is specific for stearoyl-CoA Carthamus tinctorius oleoyl-[acyl-carrier protein] + acceptor + H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Carthamus tinctorius