Cloned (Comment) | Organism |
---|---|
expressed in HeLa cells, tagged with myc epitope at the C terminus or HA epitope | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C222S | with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type | Mus musculus |
C233S | with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type | Mus musculus |
C322S | with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type | Mus musculus |
C92S | with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type | Mus musculus |
C92S/C97S/C222S/C322S | with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity 5fold increased compared with cells transfected with the empty vector, clear ER pattern | Mus musculus |
C97S | with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type | Mus musculus |
additional information | activities of enzymes with HA epitope tagged to the N terminus and Myc epitope tagged to the C terminus expressed in HeLa cells are 23fold and 39fold higher than in cells transfected with an empty vector, acitivities of enzymes with HA epitope after amino acid 93 and 138 additionally Myc epitope tagged to the C terminus are 5.5fold and 5.9fold higher than in cells transfected with an empty vector, respectively | Mus musculus |
additional information | Myc epitope at the C terminus and additional HA epitope at predicted loops, after amino acid 93, 138 or 241 | Mus musculus |
additional information | no activity when HA epitope is inserted after amino acid 241 | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | from HeLa cells expressing stearoyl-CoA 9-desaturase | Mus musculus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
Myc or HA epitope tagged proteins expressed in HeLa cells, immunoblot using anti-Myc and ant-HA antibodies | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
stearoyl-CoA + NADH + O2 | - |
Mus musculus | oleoyl-CoA + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | N and C termini are oriented toward the cytosol, loop between the first and second transmembrane domain is oriented toward the ER lumen, the loop between the second and third transmembrane domain is oriented toward the cytosol, the loop between the third and fourth transmembrane domain is oriented toward the ER lumen, indirect immunofluorescence of transiently transfected HeLa cells | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
SCD1 | - |
Mus musculus |