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Literature summary for 1.14.19.1 extracted from

  • Yang, Y.S.; Broadwater, J.A.; Pulver, S.C.; Fox, B.G.; Solomon, E.I.
    Circular dichroism and magnetic circular dichroism studies of the reduced binuclear non-heme iron site of stearoyl-ACP DELTA9-desaturase: substrate binding and comparison to ribonucleotide reductase (1999), J. Am. Chem. Soc., 121, 2770-2783.
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
coexpression of acyl carrier protein isoform I gene and holo-acyl carrier protein synthase of Escherichia coli Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Spinacia oleracea 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ each subunit of homodimer contains one binuclear non-heme iron active site. The reduced DELTA9-desaturase has two approximately equivalent 5-coordinate irons in a distorted square pyramidal geometry, a two-in-two-out equatorial distortion, the irons are rhombic, weakly antiferromagnetically coupled. Addition of substrate causes dramatic changes, first ion remains 5-coordinate but is distorted toward a trigonal bipyramidal structure, second iron changes to 4-coordinate Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 homodimer, 2 * 40000 Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
stearoyl-acyl-carrier protein + AH2 + O2 Spinacia oleracea insertion of a cis double bond between the 9 and 10 position, essential step in fatty acid biosynthesis oleoyl-acyl-carrier protein + A + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Reaction

Reaction Comment Organism Reaction ID
stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O the possible mechanism would be that dioxygen has to bind to both irons for effective two-electron transfer to the half-occupied antibonding pi orbitals of dioxygen to give a peroxide level intermediate Spinacia oleracea
a

Storage Stability

Storage Stability Organism
reduced enzyme is stable under O2 for hours Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
stearoyl-acyl-carrier protein + AH2 + O2 insertion of a cis double bond between the 9 and 10 position, essential step in fatty acid biosynthesis Spinacia oleracea oleoyl-acyl-carrier protein + A + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer homodimer, 2 * 40000 Spinacia oleracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 stearoyl-acyl-carrier protein
-
 Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Spinacia oleracea