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Literature summary for 1.14.19.1 extracted from

  • Fulco, A.J.; Bloch, K.
    Cofactor requirements for the formation of DELTA9-unsaturated fatty acids in Mycobacterium phlei (1964), J. Biol. Chem., 239, 993-997.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Mycolicibacterium phlei
CN-
-
Mycolicibacterium phlei
Cu+ strong, complete inhibition at 0.1 mM Mycolicibacterium phlei
dihydrolipoic acid
-
Mycolicibacterium phlei
EDTA
-
Mycolicibacterium phlei
menadione 85% inhibition at 0.1 mM Mycolicibacterium phlei
methylene blue
-
Mycolicibacterium phlei
additional information sulfhydryl compounds Mycolicibacterium phlei
NADH
-
Mycolicibacterium phlei
o-phenanthroline 80% inhibition at 2 mM Mycolicibacterium phlei
p-chloromercuribenzoate strong, complete inhibition at 0.02 mM Mycolicibacterium phlei
phenazine methosulfate nearly complete inhibition at 0.3 mM Mycolicibacterium phlei
riboflavin 25-30% inhibition at 0.04 mM Mycolicibacterium phlei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
FAD
-
Mycolicibacterium phlei
0.0025
-
FMN
-
Mycolicibacterium phlei
0.8
-
NADPH
-
Mycolicibacterium phlei

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ specifically required Mycolicibacterium phlei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
stearoyl-CoA + AH2 + O2 Mycolicibacterium phlei
-
oleoyl-CoA + A + H2O
-
?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium phlei
-
ATCC 356
-

Storage Stability

Storage Stability Organism
-20°C, sedimented enzyme, 0.25 M sucrose, 2 weeks, without significant loss of activity Mycolicibacterium phlei
-20°C, supernatant fraction, over 1 year, without loss of activity Mycolicibacterium phlei
4°C, 1 mM or 10 mM potassium phosphate buffer, pH 7.2, 24 hours, activity irreversibly lost Mycolicibacterium phlei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + NADPH + O2
-
Mycolicibacterium phlei ?
-
?
stearoyl-CoA + AH2 + O2
-
Mycolicibacterium phlei oleoyl-CoA + A + H2O
-
?
stearoyl-CoA + NADPH + O2
-
Mycolicibacterium phlei oleoyl-CoA + NADP+ + H2O
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mycolicibacterium phlei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
30 min in distilled water or 0.1 M phosphate buffer, pH 7.2 causes in almost complete loss of activity Mycolicibacterium phlei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Mycolicibacterium phlei

Cofactor

Cofactor Comment Organism Structure
FAD required Mycolicibacterium phlei
FMN required, almost as active as FAD Mycolicibacterium phlei
NADPH specifically required, seems not to be directly involved in the desaturation reaction but serves to keep another cofactor in the reduced state, maybe FADH Mycolicibacterium phlei