Literature summary for 1.14.18.1 extracted from

Goldfeder, M.; Egozy, M.; Shuster Ben-Yosef, V.; Adir, N.; Fishman, A.
Changes in tyrosinase specificity by ionic liquids and sodium dodecyl sulfate (2013), Appl. Microbiol. Biotechnol., 97, 1953-1961.

Search for more literature for 1.14.18.1

Activating Compound

Activating Compound	Comment	Organism	Structure
1-butyl-3-methylimidazolium tetrafluoroborate	in the presence of 1-butyl-3-methylimidazolium tetrafluoroborate the selectivity of tyrosinase is altered, and the ratio of monophenolase/diphenolase activity increases by up to 5fold	Priestia megaterium
ethylammonium nitrate	in the presence of ethylammonium nitrate the selectivity of tyrosinase is altered, and the ratio of monophenolase/diphenolase activity increases by up to 5fold	Priestia megaterium
SDS	the addition of SDS at levels of 2-50 mM increases the activity of the enzyme by 2fold towards the natural substrates L-tyrosine and L-Dopa and 15-20fold towards the non-native phenol and catechol	Priestia megaterium

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Priestia megaterium

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Priestia megaterium

Protein Variants

Protein Variants	Comment	Organism
R209H	the mutant has a 45fold increase in activity towards phenol in the presence of SDS compared to the wild type enzyme	Priestia megaterium

Inhibitors

Inhibitors	Comment	Organism	Structure
1-ethyl-3-methylimidazolium ethyl sulfate	in the presence of 10 or 20% (w/v) 1-ethyl-3-methylimidazolium ethyl sulfate, the activity decreases dramatically and becomes negligible	Priestia megaterium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	a type 3 copper protein	Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-Dopa + O2	Priestia megaterium	-	L-dopaquinone + H2O	-	?
L-tyrosine + O2	Priestia megaterium	-	L-dopaquinone + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Priestia megaterium	B2ZB02	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Priestia megaterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 catechol + O2	-	Priestia megaterium	2 1,2-benzoquinone + 2 H2O	-	?
L-Dopa + O2	-	Priestia megaterium	L-dopaquinone + H2O	-	?
L-tyrosine + O2	-	Priestia megaterium	L-dopaquinone + H2O	-	?
phenol + O2	-	Priestia megaterium	?	-	?

Synonyms

Synonyms	Comment	Organism
diphenolase	-	Priestia megaterium
monophenolase	-	Priestia megaterium
tyr	-	Priestia megaterium

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)