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Literature summary for 1.14.18.1 extracted from

  • Shuster, V.; Fishman, A.
    Isolation, cloning and characterization of a tyrosinase with improved activity in organic solvents from Bacillus megaterium (2009), J. Mol. Microbiol. Biotechnol., 17, 188-200.
    View publication on PubMed
Search for more literature for 1.14.18.1

Activating Compound

Activating Compound Comment Organism Structure
2-propanol activity is enhanced in the presence of 10-50% (v/v) Priestia megaterium
DMSO activity is enhanced in the presence of 10-50% (v/v), the activity in 30% (v/v) DMSO is 170% of the activity in water and the enantioselectivity towards L-DOPA decreases by 40% Priestia megaterium
ethanol activity is enhanced in the presence of 10-50% (v/v) Priestia megaterium
methanol activity is enhanced in the presence of 10-50% (v/v), the residual activity following an incubation period of 17 h in 0-70% methanol is constant Priestia megaterium

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Priestia megaterium

Inhibitors

Inhibitors Comment Organism Structure
beta-mercaptoethanol complete inhibition at 1.0 mM Priestia megaterium
EDTA 27% inhibition at 1.0 mM Priestia megaterium
glutathione complete inhibition at 1.0 mM Priestia megaterium
Sodium diethyldithiocarbamate complete inhibition at 1.0 mM Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
 L-tyrosine in 50 mM potassium phosphate buffer (pH 7.0) Priestia megaterium
0.35
-
 L-Dopa in 50 mM potassium phosphate buffer (pH 7.0) Priestia megaterium
0.73
-
 L-Dopa in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO Priestia megaterium
1.18
-
 D-Dopa in 50 mM potassium phosphate buffer (pH 7.0) Priestia megaterium
1.7
-
 D-Dopa in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 gel filtration Priestia megaterium
35000
-
 SDS-PAGE Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-bound affinity column chromatography, gel filtration Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-catechin + O2 60.8% activity compared to L-DOPA Priestia megaterium ?
-
 ?
(+/-)-catechin + O2 58.7% activity compared to L-DOPA Priestia megaterium ?
-
 ?
2 catechol + O2 10.3% activity compared to L-DOPA Priestia megaterium 2 1,2-benzoquinone + 2 H2O
-
 ?
3-aminophenol + O2 3.9% activity compared to L-DOPA Priestia megaterium ?
-
 ?
caffeic acid + O2 60.9% activity compared to L-DOPA Priestia megaterium caffeoyl quinone + H2O
-
 ?
chlorogenic acid + O2 20.1% activity compared to L-DOPA Priestia megaterium ?
-
 ?
D-DOPA + O2 34.0% activity compared to L-DOPA Priestia megaterium D-dopaquinone + H2O
-
 ?
D-tyrosine + O2 20.3% activity compared to L-DOPA Priestia megaterium D-dopa + H2O
-
 ?
DL-DOPA + O2 78% activity compared to L-DOPA Priestia megaterium DL-dopaquinone + H2O
-
 ?
DL-tyrosine + O2 20.6% activity compared to L-DOPA Priestia megaterium DL-DOPA + H2O
-
 ?
epicatechin + O2 23.4% activity compared to L-DOPA Priestia megaterium ?
-
 ?
L-Dopa + O2 100% activity Priestia megaterium L-dopaquinone + H2O
-
 ?
L-tyrosine + O2 35.6% activity compared to L-DOPA Priestia megaterium L-DOPA + H2O
-
 ?
additional information vanillic acid, 1-naphthol, 2,6-dimethoxyphenol, and resorcinol are no substrates for tyrosinase Priestia megaterium ?
-
 ?
o-coumaric acid + O2 less than 1% activity compared to L-DOPA Priestia megaterium ?
-
 ?
p-coumaric acid + O2 6.1% activity compared to L-DOPA Priestia megaterium caffeic acid + H2O
-
 ?
p-hydroxybenzoic acid + O2 less than 1% activity compared to L-DOPA Priestia megaterium ?
-
 ?
phenol + O2 less than 1% activity compared to L-DOPA Priestia megaterium ?
-
 ?
phloroglucin + O2 1.6% activity compared to L-DOPA Priestia megaterium ?
-
 ?
pyrogallol + O2 99.9% activity compared to L-DOPA Priestia megaterium ?
-
 ?
tyramine + O2 2.5% activity compared to L-DOPA Priestia megaterium ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 31000, gel filtration Priestia megaterium

Synonyms

Synonyms Comment Organism
tyrosinase
-
 Priestia megaterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 the maximum activity for both monophenolase and diphenolase activities of tyrosinase is observed at 50°C Priestia megaterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 70
-
 Priestia megaterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 the enzyme is fully stable below 50°C, but rapidly loses its activity at 70°C Priestia megaterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
 L-tyrosine in 50 mM potassium phosphate buffer (pH 7.0) Priestia megaterium
6
-
 D-Dopa in 50 mM potassium phosphate buffer (pH 7.0) Priestia megaterium
10.1
-
 L-Dopa in 50 mM potassium phosphate buffer (pH 7.0) Priestia megaterium
12.12
-
 D-Dopa in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO Priestia megaterium
21.3
-
 L-Dopa in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 the maximum activity for both monophenolase and diphenolase activities of tyrosinase is observed at pH 7.0 Priestia megaterium

pH Range

pH Minimum pH Maximum Comment Organism
4 7 for diphenolase activity of tyrosinase Priestia megaterium
4 10 for monophenolase activity of tyrosinase Priestia megaterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1
-
 D-Dopa in 50 mM potassium phosphate buffer (pH 7.0) Priestia megaterium
7.1
-
 D-Dopa in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO Priestia megaterium
28.9
-
 L-Dopa in 50 mM potassium phosphate buffer (pH 7.0) Priestia megaterium
29.2
-
 L-Dopa in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO Priestia megaterium
32.9
-
 L-tyrosine in 50 mM potassium phosphate buffer (pH 7.0) Priestia megaterium