Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-propanol | activity is enhanced in the presence of 10-50% (v/v) | Priestia megaterium | |
DMSO | activity is enhanced in the presence of 10-50% (v/v), the activity in 30% (v/v) DMSO is 170% of the activity in water and the enantioselectivity towards L-DOPA decreases by 40% | Priestia megaterium | |
ethanol | activity is enhanced in the presence of 10-50% (v/v) | Priestia megaterium | |
methanol | activity is enhanced in the presence of 10-50% (v/v), the residual activity following an incubation period of 17 h in 0-70% methanol is constant | Priestia megaterium |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Priestia megaterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | complete inhibition at 1.0 mM | Priestia megaterium | |
EDTA | 27% inhibition at 1.0 mM | Priestia megaterium | |
glutathione | complete inhibition at 1.0 mM | Priestia megaterium | |
Sodium diethyldithiocarbamate | complete inhibition at 1.0 mM | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
L-tyrosine | in 50 mM potassium phosphate buffer (pH 7.0) | Priestia megaterium | |
0.35 | - |
L-Dopa | in 50 mM potassium phosphate buffer (pH 7.0) | Priestia megaterium | |
0.73 | - |
L-Dopa | in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO | Priestia megaterium | |
1.18 | - |
D-Dopa | in 50 mM potassium phosphate buffer (pH 7.0) | Priestia megaterium | |
1.7 | - |
D-Dopa | in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
gel filtration | Priestia megaterium |
35000 | - |
SDS-PAGE | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-bound affinity column chromatography, gel filtration | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-catechin + O2 | 60.8% activity compared to L-DOPA | Priestia megaterium | ? | - |
? | |
(+/-)-catechin + O2 | 58.7% activity compared to L-DOPA | Priestia megaterium | ? | - |
? | |
2 catechol + O2 | 10.3% activity compared to L-DOPA | Priestia megaterium | 2 1,2-benzoquinone + 2 H2O | - |
? | |
3-aminophenol + O2 | 3.9% activity compared to L-DOPA | Priestia megaterium | ? | - |
? | |
caffeic acid + O2 | 60.9% activity compared to L-DOPA | Priestia megaterium | caffeoyl quinone + H2O | - |
? | |
chlorogenic acid + O2 | 20.1% activity compared to L-DOPA | Priestia megaterium | ? | - |
? | |
D-DOPA + O2 | 34.0% activity compared to L-DOPA | Priestia megaterium | D-dopaquinone + H2O | - |
? | |
D-tyrosine + O2 | 20.3% activity compared to L-DOPA | Priestia megaterium | D-dopa + H2O | - |
? | |
DL-DOPA + O2 | 78% activity compared to L-DOPA | Priestia megaterium | DL-dopaquinone + H2O | - |
? | |
DL-tyrosine + O2 | 20.6% activity compared to L-DOPA | Priestia megaterium | DL-DOPA + H2O | - |
? | |
epicatechin + O2 | 23.4% activity compared to L-DOPA | Priestia megaterium | ? | - |
? | |
L-Dopa + O2 | 100% activity | Priestia megaterium | L-dopaquinone + H2O | - |
? | |
L-tyrosine + O2 | 35.6% activity compared to L-DOPA | Priestia megaterium | L-DOPA + H2O | - |
? | |
additional information | vanillic acid, 1-naphthol, 2,6-dimethoxyphenol, and resorcinol are no substrates for tyrosinase | Priestia megaterium | ? | - |
? | |
o-coumaric acid + O2 | less than 1% activity compared to L-DOPA | Priestia megaterium | ? | - |
? | |
p-coumaric acid + O2 | 6.1% activity compared to L-DOPA | Priestia megaterium | caffeic acid + H2O | - |
? | |
p-hydroxybenzoic acid + O2 | less than 1% activity compared to L-DOPA | Priestia megaterium | ? | - |
? | |
phenol + O2 | less than 1% activity compared to L-DOPA | Priestia megaterium | ? | - |
? | |
phloroglucin + O2 | 1.6% activity compared to L-DOPA | Priestia megaterium | ? | - |
? | |
pyrogallol + O2 | 99.9% activity compared to L-DOPA | Priestia megaterium | ? | - |
? | |
tyramine + O2 | 2.5% activity compared to L-DOPA | Priestia megaterium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31000, gel filtration | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
tyrosinase | - |
Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the maximum activity for both monophenolase and diphenolase activities of tyrosinase is observed at 50°C | Priestia megaterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 70 | - |
Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | the enzyme is fully stable below 50°C, but rapidly loses its activity at 70°C | Priestia megaterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
L-tyrosine | in 50 mM potassium phosphate buffer (pH 7.0) | Priestia megaterium | |
6 | - |
D-Dopa | in 50 mM potassium phosphate buffer (pH 7.0) | Priestia megaterium | |
10.1 | - |
L-Dopa | in 50 mM potassium phosphate buffer (pH 7.0) | Priestia megaterium | |
12.12 | - |
D-Dopa | in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO | Priestia megaterium | |
21.3 | - |
L-Dopa | in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
the maximum activity for both monophenolase and diphenolase activities of tyrosinase is observed at pH 7.0 | Priestia megaterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | for diphenolase activity of tyrosinase | Priestia megaterium |
4 | 10 | for monophenolase activity of tyrosinase | Priestia megaterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.1 | - |
D-Dopa | in 50 mM potassium phosphate buffer (pH 7.0) | Priestia megaterium | |
7.1 | - |
D-Dopa | in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO | Priestia megaterium | |
28.9 | - |
L-Dopa | in 50 mM potassium phosphate buffer (pH 7.0) | Priestia megaterium | |
29.2 | - |
L-Dopa | in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO | Priestia megaterium | |
32.9 | - |
L-tyrosine | in 50 mM potassium phosphate buffer (pH 7.0) | Priestia megaterium |