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Literature summary for 1.14.18.1 extracted from
- Partial purification of latent polyphenol oxidase from peach (Prunus persica L. Cv. Catherina). Molecular properties and kinetic characterization of soluble and membrane-bound forms (2007), J. Agric. Food Chem., 55, 10446-10451.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| SDS | soluble and membrane-bound peach polyphenol oxidase are activated by acid shock and the detergent SDS | Prunus persica |  |
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | PPO is a very important enzyme in the food industry because during the processing of fruits and vegetables any wounding may cause cell disruption and lead to the formation of quinones, and their interaction with amino acids and proteins will enhance the brown color produced | Prunus persica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.263 | - |
4-tert-butylcatechol | soluble (PPO-S) peach polyphenol oxidase without SDS | Prunus persica | |
| 0.3 | - |
4-tert-butylcatechol | membrane-bound (PPO-B) peach polyphenol oxidase without SDS | Prunus persica | |
| 0.85 | - |
4-tert-butylcatechol | membrane-bound (PPO-B) peach polyphenol oxidase with 2 mM SDS | Prunus persica | |
| 1.84 | - |
4-tert-butylcatechol | soluble (PPO-S) peach polyphenol oxidase with 2 mM SDS | Prunus persica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Prunus persica | 16020 | - |
| soluble | - |
Prunus persica | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
a partially denaturing SDS-PAGE reveals two very close bands of activity in both cases, but the Western blot performed on a totally denaturing SDS-PAGE, using polyclonal antibodies against bean PPO, reveals a single band in the membrane-bound fraction with a molecular mass of 60 kDa | Prunus persica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prunus persica | - |
Prunus persica L. Cv. Catherina | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification, the membrane-bound PPO is extracted by solubilization with the detergent Triton X-114 followed by temperature-induced phase separation | Prunus persica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Prunus persica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.063 | - |
PPO-s soluble, crude extract | Prunus persica |
| 0.079 | - |
PPO-s soluble, supernatant 120000g | Prunus persica |
| 0.096 | - |
PPO-B membrane bound, 6% Triton-X-114 | Prunus persica |
| 0.22 | - |
PPO-B membrane bound, ammoniun sulfate 25-80% | Prunus persica |
| 0.45 | - |
PPO-s soluble, ammoniun sulfate 25-80% | Prunus persica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-tert-butylcatechol + 1/2 O2 | - |
Prunus persica | 4-(tert-butyl)benzo-1,2-quinone + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| monophenol, o-diphenol: oxygen oxidoreductase | - |
Prunus persica |
| polyphenol oxidase | - |
Prunus persica |
| PPO | - |
Prunus persica |
| tyrosinase | - |
Prunus persica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Prunus persica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Prunus persica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7.5 | enzyme activity toward 5 mM 4-tert-butylcatechol is determined in a range of pH from 3.5 to 7.5 | Prunus persica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Prunus persica | native isoelectric focusing identifies two acid isoforms of pI 5.7 and 5.8 for the soluble form and one isoform with pI 5.7 for the membrane-bound form | - |
5.7 |
| Prunus persica | native isoelectric focusing identifies two acid isoforms of pI 5.7 and 5.8 for the soluble form and one isoform with pI 5.7 for the membrane-bound form | - |
5.8 |
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