Application | Comment | Organism |
---|---|---|
pharmacology | study of suicide inactivation and irreversible inhibition is important in the functional design of synthetic inactivators for therapeutic applications | Agaricus bisporus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | suicide inactivation of tyrosinase acting on o-diphenols. A kinetic study of the suicide inactivation of tyrosinase during its action on a variety of substrates is described, and a mechanism is proposed to explain the experimental kinetic results and to throw light on the suicide inactivation step of mushroom tyrosinase | Agaricus bisporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
protocatechuic acid | - |
Agaricus bisporus | |
0.1 | - |
4-methylcatechol | - |
Agaricus bisporus | |
0.1 | - |
4-nitrocatechol | - |
Agaricus bisporus | |
0.12 | - |
methyl gallate | - |
Agaricus bisporus | |
0.15 | - |
Protocatechuic aldehyde | - |
Agaricus bisporus | |
0.16 | - |
catechol | - |
Agaricus bisporus | |
0.17 | - |
4-Ethylcatechol | - |
Agaricus bisporus | |
0.18 | - |
gallic acid | - |
Agaricus bisporus | |
0.6 | - |
4-Chlorocatechol | - |
Agaricus bisporus | |
0.7 | - |
3,4-Dihydroxyphenylpropionic acid | - |
Agaricus bisporus | |
1.3 | - |
3,4-dihydroxyphenylacetic acid | - |
Agaricus bisporus | |
1.45 | - |
4-tert-butylcatechol | - |
Agaricus bisporus | |
1.96 | - |
pyrogallol | - |
Agaricus bisporus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | - |
Agaricus bisporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Agaricus bisporus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Agaricus bisporus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8300 | - |
mushroom tyrosinase 8300 U/mg | Agaricus bisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetic acid + 1/2 O2 | DHPAA | Agaricus bisporus | (3,4-dioxocyclohexa-1,5-dien-1-yl)acetic acid + H2O | - |
? | |
3,4-dihydroxyphenylpropionic acid + 1/2 O2 | DHPPA | Agaricus bisporus | 3-(3,4-dioxocyclohexa-1,5-dien-1-yl)propanoic acid + H2O | - |
? | |
4-chlorocatechol + 1/2 O2 | - |
Agaricus bisporus | 4-chlorocyclohexa-3,5-diene-1,2-dione + H2O | - |
? | |
4-ethylcatechol + 1/2 O2 | - |
Agaricus bisporus | 4-ethylcyclohexa-3,5-diene-1,2-dione + H2O | - |
? | |
4-methylcatechol + 1/2 O2 | - |
Agaricus bisporus | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
4-nitrocatechol + 1/2 O2 | - |
Agaricus bisporus | 4-nitrocyclohexa-3,5-diene-1,2-dione + H2O | - |
? | |
4-tert-butylcatechol + 1/2 O2 | - |
Agaricus bisporus | 4-tert-butylcyclohexa-3,5-diene-1,2-dione + H2O | - |
? | |
catechol + 1/2 O2 | pyrogallol and catechol are best substrates for catalysis and inactivation | Agaricus bisporus | 1,2-benzoquinone + H2O | - |
? | |
gallic acid + 1/2 O2 | - |
Agaricus bisporus | 5-hydroxy-3,4-dioxocyclohexa-1,5-diene-1-carboxylic acid + H2O | - |
? | |
methyl gallate + O2 | - |
Agaricus bisporus | methyl 5-hydroxy-3,4-dioxocyclohexa-1,5-diene-1-carboxylate + H2O | - |
? | |
protocatechuic acid + 1/2 O2 | - |
Agaricus bisporus | 3,4-dioxocyclohexa-1,5-diene-1-carboxylic acid + H2O | - |
? | |
protocatechuic aldehyde + 1/2 O2 | - |
Agaricus bisporus | 3,4-dioxocyclohexa-1,5-diene-1-carbaldehyde + H2O | - |
? | |
pyrogallol + 1/2 O2 | pyrogallol and catechol are best substrates for catalysis and inactivation | Agaricus bisporus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
o-diphenol: oxidoreductase | - |
Agaricus bisporus |
polyphenol oxidase | - |
Agaricus bisporus |
tyrosinase | - |
Agaricus bisporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Agaricus bisporus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
4-nitrocatechol | - |
Agaricus bisporus | |
1.5 | - |
Protocatechuic aldehyde | - |
Agaricus bisporus | |
8.1 | - |
protocatechuic acid | - |
Agaricus bisporus | |
28.2 | - |
gallic acid | - |
Agaricus bisporus | |
80.2 | - |
methyl gallate | - |
Agaricus bisporus | |
433.1 | - |
3,4-dihydroxyphenylacetic acid | - |
Agaricus bisporus | |
607.2 | - |
3,4-Dihydroxyphenylpropionic acid | - |
Agaricus bisporus | |
640.1 | - |
4-tert-butylcatechol | - |
Agaricus bisporus | |
802.3 | - |
4-Ethylcatechol | - |
Agaricus bisporus | |
842.1 | - |
4-methylcatechol | - |
Agaricus bisporus | |
859.2 | - |
4-Chlorocatechol | - |
Agaricus bisporus | |
874.1 | - |
catechol | - |
Agaricus bisporus | |
1280 | - |
pyrogallol | - |
Agaricus bisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Agaricus bisporus |