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Literature summary for 1.14.18.1 extracted from

  • Kong, K.H.; Hong, M.P.; Choi, S.S.; Kim, Y.T.; Cho, S.H.
    Purification and characterization of a highly stable tyrosinase from Thermomicrobium roseum (2000), Biotechnol. Appl. Biochem., 31, 113-118.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 0.1 mM, complete inhibition Thermomicrobium roseum
5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one 1 mM, 50% inhibition of L-dopa oxidation Thermomicrobium roseum
Cl- 1 mM, 35% inhibition of L-dopa oxidation Thermomicrobium roseum
diethyldithiocarbamate 1 mM, complete inhibition of L-dopa oxidation Thermomicrobium roseum
EDTA 1 mM, 71% inhibition of L-dopa oxidation Thermomicrobium roseum
glutathione 1 mM, 86% inhibition of L-dopa oxidation Thermomicrobium roseum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
L-Dopa
-
Thermomicrobium roseum

Metals/Ions

Metals/Ions Comment Organism Structure
copper 50% activation of L-dopa oxidation Thermomicrobium roseum
K+ 50% activation of L-dopa oxidation Thermomicrobium roseum
Mg2+ 33% activation of L-dopa oxidation Thermomicrobium roseum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, SDS-PAGE Thermomicrobium roseum
90000
-
gel filtration Thermomicrobium roseum

Organism

Organism UniProt Comment Textmining
Thermomicrobium roseum
-
ATCC 27502
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel, Mono Q Thermomicrobium roseum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.49
-
-
Thermomicrobium roseum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(RS)-catechin + O2 83% of activity with L-dopa Thermomicrobium roseum ?
-
?
4-hydroxybenzoic acid + O2 + AH2 50% of activity with L-dopa Thermomicrobium roseum 3,4-dihydroxybenzoic acid + H2O + A
-
?
caffeic acid + 1/2 O2 17% of activity with L-dopa Thermomicrobium roseum caffeoyl quinone + H2O
-
?
catechol + 1/2 O2 125% of activity with L-dopa Thermomicrobium roseum 1,2-benzoquinone + H2O
-
?
chlorogenic acid + O2 108% of activity with L-dopa Thermomicrobium roseum chlorogenoquinone + H2O
-
?
D-catechin + O2 67% of activity with L-dopa Thermomicrobium roseum ?
-
?
DL-epicatechin + O2 91% of activity with L-dopa Thermomicrobium roseum ?
-
?
dopa + 1/2 O2
-
Thermomicrobium roseum dopaquinone + H2O
-
?
L-tyrosine + O2 + AH2 10% of activity with L-dopa Thermomicrobium roseum L-dopa + H2O + A
-
?
o-coumaric acid + O2 58% of activity with L-dopa Thermomicrobium roseum ?
-
?
orcin + O2 42% of activity with L-dopa Thermomicrobium roseum ?
-
?
phloroglucin + O2 87% of activity with L-dopa Thermomicrobium roseum ?
-
?
pyrogallol + O2 417% of activity with L-dopa Thermomicrobium roseum ?
-
?
resorcinol + O2 67% of activity with L-dopa Thermomicrobium roseum ?
-
?
tyramine + O2 67% of activity with L-dopa Thermomicrobium roseum 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE Thermomicrobium roseum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Thermomicrobium roseum

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10.5 less than 50% activity below pH 7.5 and above pH 10.5, L-dopa oxidation at 50°C Thermomicrobium roseum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
4°C, 20 h, about 75% loss of activity below Thermomicrobium roseum
8.5 10 4°C, 20 h, enzyme retains more than 70% of activity in the pH-range 8.5-10.0 Thermomicrobium roseum
11
-
4°C, 20 h, about 75% loss of activity above Thermomicrobium roseum