Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-ascorbic acid | 0.04 mM, complete inhibition | Pinus densiflora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.7 | - |
L-Dopa | isoenzyme 1 | Pinus densiflora | |
9.3 | - |
L-Dopa | isoenzyme 2 | Pinus densiflora | |
10 | - |
L-Dopa | isoenzyme 3 | Pinus densiflora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | 1 mM, increase in activity | Pinus densiflora | |
Fe3+ | 1 mM, increase in activity | Pinus densiflora | |
Mg2+ | 1 mM, increase in activity | Pinus densiflora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, isoenzyme 3, SDS-PAGE | Pinus densiflora |
55000 | - |
x * 55000, isoenzyme 2, SDS-PAGE | Pinus densiflora |
65000 | - |
x * 65000, isoenzyme 1, SDS-PAGE | Pinus densiflora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pinus densiflora | - |
3 isoenzymes | - |
Purification (Comment) | Organism |
---|---|
Sephacryl S-200, Sephadex G-75, DEAE-Sephacel, isoenzymes 1-3 | Pinus densiflora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(RS)-catechin + O2 | isoenzymes 1-3, 96, 104, and 168% of activity with L-dopa respectively | Pinus densiflora | ? | - |
? | |
3,4-dihydroxybenzoic acid + 1/2 O2 | isoenzymes 1-3, 22%, 13%, and 13% of L-dopa activity respectively | Pinus densiflora | (3,4-dioxocyclohexa-1,5-dien-1-yl)acetic acid + H2O | - |
? | |
caffeic acid + 1/2 O2 | - |
Pinus densiflora | caffeoyl quinone + H2O | - |
? | |
catechol + 1/2 O2 | - |
Pinus densiflora | 1,2-benzoquinone + H2O | - |
? | |
chlorogenic acid + O2 | - |
Pinus densiflora | chlorogenoquinone + H2O | - |
? | |
D-catechin + O2 | isoenzymes 1, 2 and 3, 90%, 86% and 188% of L-dopa activity respectively | Pinus densiflora | ? | - |
? | |
dopa + 1/2 O2 | - |
Pinus densiflora | dopaquinone + H2O | - |
? | |
L-epicatechin + O2 | isoenzyme 1, 150% of L-dopa activity, isoenzyme 2 and 3, 170% and 175% of activity with L-dopa respectively | Pinus densiflora | ? | - |
? | |
orcin + O2 | isoenzymes 1-3, 41%, 33%, and 25% of activity with L-dopa respectively | Pinus densiflora | ? | - |
? | |
p-coumaric acid + O2 + AH2 | - |
Pinus densiflora | caffeic acid + H2O + A | - |
? | |
pyrogallol + O2 | isoenzymes 1-3, 210%, 263%, and 225% of activity with L-dopa respectively | Pinus densiflora | ? | - |
? | |
resorcinol + O2 | isoenzymes 1-3, 96%, 129%, and 100% of activity with L-dopa respectively | Pinus densiflora | ? | - |
? | |
tyramine + O2 | isoenzymes 1, 20% of L-dopa activity, isoenzyme2, 13% of activity with L-dopa, isoenzyme 3, 25% of activity with L-dopa | Pinus densiflora | 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, isoenzyme 2, SDS-PAGE | Pinus densiflora |
? | x * 45000, isoenzyme 3, SDS-PAGE | Pinus densiflora |
? | x * 65000, isoenzyme 1, SDS-PAGE | Pinus densiflora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | isoenzymes 1-3 | Pinus densiflora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.6 | isoenzymes 1-3 | Pinus densiflora |