Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, complete inhibition | Dioscorea cayenensis subsp. rotundata | |
CN- | 1 mM, 96% inhibition | Dioscorea cayenensis subsp. rotundata | |
diethyldithiocarbamate | 1 mM, complete inhibition | Dioscorea cayenensis subsp. rotundata | |
DL-dithiothreitol | 1 mM, complete inhibition | Dioscorea cayenensis subsp. rotundata | |
iodobenzoic acid | 1 mM, 78% inhibition | Dioscorea cayenensis subsp. rotundata | |
L-ascorbic acid | 1 mM, complete inhibition of purified enzyme, 20% inhibition of crude enzyme | Dioscorea cayenensis subsp. rotundata | |
L-cysteine | 1 mM, complete inhibition | Dioscorea cayenensis subsp. rotundata | |
Metabisulfite | 1 mM, complete inhibition | Dioscorea cayenensis subsp. rotundata | |
N3- | - |
Dioscorea cayenensis subsp. rotundata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
catechol | - |
Dioscorea cayenensis subsp. rotundata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | 4 gatom per mol | Dioscorea cayenensis subsp. rotundata | |
copper | 0.22% copper, probably 4 copper atoms per enzyme molecule | Dioscorea cayenensis subsp. rotundata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
107000 | - |
gel filtration | Dioscorea cayenensis subsp. rotundata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dioscorea cayenensis subsp. rotundata | - |
white yam from Nigeria | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sephadex, Sephadex G-20, DEAE-Sephadex | Dioscorea cayenensis subsp. rotundata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tuber | - |
Dioscorea cayenensis subsp. rotundata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
9900 units, 1 unit is defined as the amount of enzyme that causes a change in absorbance of 0.001/min | Dioscorea cayenensis subsp. rotundata |
Storage Stability | Organism |
---|---|
4°C, 2 weeks, 10% loss of activity | Dioscorea cayenensis subsp. rotundata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + 1/2 O2 | - |
Dioscorea cayenensis subsp. rotundata | 1,2-benzoquinone + H2O | - |
? | |
chlorogenic acid + O2 | - |
Dioscorea cayenensis subsp. rotundata | chlorogenoquinone + H2O | - |
? | |
dopa + 1/2 O2 | - |
Dioscorea cayenensis subsp. rotundata | dopaquinone + H2O | - |
? | |
pyrogallol + O2 | - |
Dioscorea cayenensis subsp. rotundata | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Dioscorea cayenensis subsp. rotundata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, stable | Dioscorea cayenensis subsp. rotundata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Dioscorea cayenensis subsp. rotundata |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
L-cysteine | - |
Dioscorea cayenensis subsp. rotundata | |
0.2 | - |
CN- | - |
Dioscorea cayenensis subsp. rotundata | |
0.5 | - |
2-mercaptoethanol | - |
Dioscorea cayenensis subsp. rotundata | |
1.8 | - |
N3- | - |
Dioscorea cayenensis subsp. rotundata |