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Literature summary for 1.14.18.1 extracted from
- Isolation and characterization of the polyphenoloxidase from senescent leaves of black poplar (1984), Phytochemistry, 23, 501-505.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ferulic acid | activation | Populus nigra |  |
| gallic acid | activation | Populus nigra | |
| phosphate | increase of activity | Populus nigra | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM, inactivation, half-life: 30 min | Populus nigra | |
| 2,5-dihydroxybenzoic acid | - |
Populus nigra | |
| 2-hydroxybenzoic acid | - |
Populus nigra | |
| 3,4-dihydroxybenzoic acid | - |
Populus nigra | |
| 3-hydroxycinnamic acid | - |
Populus nigra | |
| 4-hydroxybenzoic acid | - |
Populus nigra | |
| 4-hydroxycinnamic acid | - |
Populus nigra | |
| CN- | 0.6 mM, 50% inhibition | Populus nigra | |
| Cu2+ | inhibition at concentrations higher than 5 mM | Populus nigra | |
| ferulic acid | - |
Populus nigra | |
| gallic acid | - |
Populus nigra | |
| hydroquinone | - |
Populus nigra | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
catechol | - |
Populus nigra | |
| 5 | - |
4-methylcatechol | - |
Populus nigra | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 50 mM, 360% activation | Populus nigra | |
| copper | activation at low concentrations, inhibition above 5 mM | Populus nigra | |
| Mn2+ | activation | Populus nigra | |
| Ni2+ | 50 mM, 20% activation | Populus nigra | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
PAGE | Populus nigra |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus nigra | - |
black poplar | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Sephadex G-75, DEAE-cellulose | Populus nigra |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Populus nigra | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 hydroquinone + O2 | - |
Populus nigra | 2 quinone + 2 H2O | - |
? | |
| 4-methylcatechol + O2 | - |
Populus nigra | 4-methyl-o-benzoquinone + H2O | - |
? | |
| caffeic acid + 1/2 O2 | - |
Populus nigra | caffeoyl quinone + H2O | - |
? | |
| catechol + 1/2 O2 | - |
Populus nigra | 1,2-benzoquinone + H2O | - |
? | |
| L-tyrosine + O2 + AH2 | no activity with tyrosine | Populus nigra | L-dopa + H2O + A | - |
? | |
| p-coumaric acid + O2 + AH2 | - |
Populus nigra | caffeic acid + H2O + A | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Populus nigra |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
phenol oxidase activity | Populus nigra |
| 7.5 | - |
o-diphenolase | Populus nigra |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
hydroquinone | - |
Populus nigra | |
| 0.24 | - |
4-hydroxycinnamic acid | - |
Populus nigra | |
| 0.35 | - |
2,5-dihydroxybenzoic acid | - |
Populus nigra | |
| 1.75 | - |
2-hydroxybenzoic acid | - |
Populus nigra | |
| 2 | - |
4-hydroxybenzoic acid | - |
Populus nigra | |
| 2.5 | - |
3-hydroxycinnamic acid | - |
Populus nigra | |
| 4.3 | - |
3,4-dihydroxybenzoic acid | - |
Populus nigra | |
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