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Literature summary for 1.14.18.1 extracted from

  • Yoshimoto, T.; Yamamoto, K.; Tsuru, D.
    Extracellular tyrosinase from Streptomyces sp. KY-453: purification and some enzymatic properties (1985), J. Biochem., 97, 1747-1754.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one trivial name kojic acid, 1 mM, complete inhibition of L-dopa oxidation, 94% inhibition of tyrosinase activity Streptomyces sp.
diethyldithiocarbamate 1 mM, complete inhibition of L-dopa oxidation, 21% inhibition of tyrosinase activity Streptomyces sp.
Hg2+ inhibition of tyrosine hydroxylation Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.69
-
phenol
-
Streptomyces sp.
1.11
-
pyrogallol
-
Streptomyces sp.
3
-
tyrosine
-
Streptomyces sp.
4
-
Dopa
-
Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular less than 3% intracellular activity Streptomyces sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
copper 1 gatom per mol Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000 36000 gel filtration, SDS-PAGE Streptomyces sp.
36000
-
gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
KY-453, isolated from soil
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, CM-cellulose, Sephadex G-100 Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
215
-
-
Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dopa + 1/2 O2
-
Streptomyces sp. dopaquinone + H2O
-
?
L-tyrosine + O2 + AH2
-
Streptomyces sp. L-dopa + H2O + A
-
?
phenol + O2 + AH2
-
Streptomyces sp. o-dihydroxybenzene + H2O + A
-
?
phenol + O2 + AH2
-
Streptomyces sp. catechol + H2O + A
-
?
pyrogallol + O2
-
Streptomyces sp. ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 29000-36000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
with L-dopa Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2
-
phenol
-
Streptomyces sp.
7.2
-
L-tyrosine
-
Streptomyces sp.
9.7
-
pyrogallol
-
Streptomyces sp.
118
-
L-Dopa
-
Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
with L-dopa Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9.5 80% activity after 1 h incubation Streptomyces sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00034
-
diethyldithiocarbamate non-competitive vs. L-dopa Streptomyces sp.
0.0062
-
diethyldithiocarbamate non-competitive vs. L-tyrosine Streptomyces sp.
0.013
-
5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one non-competitive vs. L-dopa Streptomyces sp.
0.057
-
5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one non-competitive vs. L-tyrosine Streptomyces sp.