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Literature summary for 1.14.18.1 extracted from

  • Wittenberg, C.; Triplett, E.L.
    A detergent-activated tyrosinase from Xenopus laevis. I. Purification and partial characterization (1985), J. Biol. Chem., 260, 12535-12541.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
SDS 3.0 mM, stimulates most effective of the anionic detergents tested Xenopus laevis
sodium deoxycholate approx. 60% activation Xenopus laevis
sodium dodecyl deoxycholate 3.0 mM, stimulates Xenopus laevis
sodium dodecyl sarcosinate 3.0 mM, stimulates Xenopus laevis
sodium dodecylsarcosinate approx. 70% activation Xenopus laevis
sodium dodecylsulfate 1 mM, 90% activation Xenopus laevis

Inhibitors

Inhibitors Comment Organism Structure
5-hydroxyindole
-
 Xenopus laevis
5-hydroxytryptophan
-
 Xenopus laevis
CN- noncompetitive vs. dopa and tyrosine Xenopus laevis
diethyldithiocarbamate noncompetitive vs. dopa and tyrosine Xenopus laevis
additional information not inhibited by 2,2'-dipyridyl Xenopus laevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 L-tyrosine
-
 Xenopus laevis
0.6
-
 Dopa
-
 Xenopus laevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
175000
-
 1 * 175000, SDS-PAGE Xenopus laevis
175000 230000 SDS-PAGE in the presence of urea Xenopus laevis
220000 229000 gel filtration in the absence and presence of SDS Xenopus laevis

Organism

Organism UniProt Comment Textmining
Xenopus laevis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Xenopus laevis

Purification (Commentary)

Purification (Comment) Organism
-
 Xenopus laevis

Source Tissue

Source Tissue Comment Organism Textmining
skin
-
 Xenopus laevis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.43
-
 tyrosine hydroxylase activity Xenopus laevis
10.4
-
 dopa oxidation Xenopus laevis

Storage Stability

Storage Stability Organism
liquid N2 or -70°C, phosphate buffer pH 7.2, 150 mM NaCl, several months, no loss of activity Xenopus laevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Dopa + O2
-
 Xenopus laevis dopaquinone + H2O
-
 r
L-tyrosine + O2
-
 Xenopus laevis dopaquinone + H2O
-
 r
L-tyrosine + O2
-
 Xenopus laevis L-DOPA + H2O
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 175000, SDS-PAGE Xenopus laevis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.33
-
 L-tyrosine tyrosine hydroxylase activity Xenopus laevis
26.7
-
 Dopa dopa oxidase activity Xenopus laevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7.3 dopa oxidase activity Xenopus laevis
6.8 7.8 tyrosine hydroxylase activity Xenopus laevis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.005
-
 5-hydroxyindole dopa oxidase inhibition Xenopus laevis
0.03
-
 diethyldithiocarbamate dopa oxidase inhibition Xenopus laevis
0.03
-
 diethyldithiocarbamate tyrosine hydroxylase Xenopus laevis
0.08
-
 CN- tyrosine hydroxylase inhibition Xenopus laevis
0.09
-
 CN- dopa oxidase inhibition Xenopus laevis