General Stability | Organism |
---|---|
highly resistant to SDS and proteinase K | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
L-Dopa | - |
Mus musculus | |
0.5 | - |
L-Dopa | in B16/C3 tumor cell homogenates | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, isoenzyme 1, immunoblot | Mus musculus |
68000 | - |
x * 68000, isoenzyme 2, immunoblot | Mus musculus |
70000 | - |
x * 70000, isoenzyme 4, immunoblot | Mus musculus |
80000 | - |
x * 80000, isoenzyme 4, immunoblot | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-3,4-dihydroxyphenylalanine + 1/2 O2 | Mus musculus | - |
L-dopaquinone + H2O | - |
? | |
L-tyrosine + O2 + AH2 | Mus musculus | rate-limiting enzyme in melanin biosynthesis | L-3,4-dihydroxyphenylalanine + H2O + A | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
B16 melanoma cells | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | isoenzymes with molecular weights of 58000 Da and 68000 Da are highly resistant to proteinase K digestion | Mus musculus |
Purification (Comment) | Organism |
---|---|
proteinase K digest, DEAE-52 cellulose, 4 isozymes | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
melanoma cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6889 | - |
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylcatechol + O2 | - |
Mus musculus | 4-methyl-o-benzoquinone + H2O | - |
? | |
dopa + 1/2 O2 | L-dopa | Mus musculus | dopaquinone + H2O | - |
? | |
L-3,4-dihydroxyphenylalanine + 1/2 O2 | - |
Mus musculus | L-dopaquinone + H2O | - |
? | |
L-tyrosine + O2 + AH2 | rate-limiting enzyme in melanin biosynthesis | Mus musculus | L-3,4-dihydroxyphenylalanine + H2O + A | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, isoenzyme 4, immunoblot | Mus musculus |
? | x * 70000, isoenzyme 4, immunoblot | Mus musculus |
? | x * 66000, isoenzyme 1, immunoblot | Mus musculus |
? | x * 68000, isoenzyme 2, immunoblot | Mus musculus |