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Literature summary for 1.14.18.1 extracted from

  • Yurkow, E.J.; Laskin, J.D.
    Purification of tyrosinase to homogeneity based on its resistance to sodium dodecyl sulfate-proteinase K digestion (1989), Arch. Biochem. Biophys., 275, 122-129.
    View publication on PubMed
Search for more literature for 1.14.18.1

General Stability

General Stability Organism
highly resistant to SDS and proteinase K Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 L-Dopa
-
 Mus musculus
0.5
-
 L-Dopa in B16/C3 tumor cell homogenates Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 x * 66000, isoenzyme 1, immunoblot Mus musculus
68000
-
 x * 68000, isoenzyme 2, immunoblot Mus musculus
70000
-
 x * 70000, isoenzyme 4, immunoblot Mus musculus
80000
-
 x * 80000, isoenzyme 4, immunoblot Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-3,4-dihydroxyphenylalanine + 1/2 O2 Mus musculus
-
 L-dopaquinone + H2O
-
 ?
L-tyrosine + O2 + AH2 Mus musculus rate-limiting enzyme in melanin biosynthesis L-3,4-dihydroxyphenylalanine + H2O + A
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 B16 melanoma cells
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information isoenzymes with molecular weights of 58000 Da and 68000 Da are highly resistant to proteinase K digestion Mus musculus

Purification (Commentary)

Purification (Comment) Organism
proteinase K digest, DEAE-52 cellulose, 4 isozymes Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
melanoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6889
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + O2
-
 Mus musculus 4-methyl-o-benzoquinone + H2O
-
 ?
dopa + 1/2 O2 L-dopa Mus musculus dopaquinone + H2O
-
 ?
L-3,4-dihydroxyphenylalanine + 1/2 O2
-
 Mus musculus L-dopaquinone + H2O
-
 ?
L-tyrosine + O2 + AH2 rate-limiting enzyme in melanin biosynthesis Mus musculus L-3,4-dihydroxyphenylalanine + H2O + A
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, isoenzyme 4, immunoblot Mus musculus
? x * 70000, isoenzyme 4, immunoblot Mus musculus
? x * 66000, isoenzyme 1, immunoblot Mus musculus
? x * 68000, isoenzyme 2, immunoblot Mus musculus