Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetate | - |
Gallus gallus | |
dehydroascorbate | - |
Gallus gallus | |
fumarate | - |
Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Gallus gallus | |
norepinephrine | - |
Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
dopamine | - |
Gallus gallus | |
2 | - |
ascorbate | - |
Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromaffin granule | - |
Gallus gallus | 42583 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | - |
Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
4 * 80000, SDS-PAGE after treatment with 2-mercaptoethanol, subunits joined in pairs by disulfide bonds | Gallus gallus |
316000 | - |
gel filtration | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Gallus gallus |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dopamine + 2 ascorbate + O2 = noradrenaline + 2 monodehydroascorbate + H2O | uni-uni bi-uni ping pong mechanism | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Gallus gallus | - |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 6 weeks | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenethylamine + ascorbate + O2 | 3,4-dihydroxyphenethylamine is identical with 4-(2-aminoethyl)-1,2-benzendiol i.e. dopamine, ferricyanide can replace ascorbate | Gallus gallus | noradrenaline + dehydroascorbate + H2O | norepinephrine | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 80000, SDS-PAGE after treatment with 2-mercaptoethanol, subunits joined in pairs by disulfide bonds | Gallus gallus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
4 | 24 h, 10-15% loss of activity | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Gallus gallus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | sharp decrease in activity between pH 4.5 and 5.0 and between pH 6.0 and 6.5 | Gallus gallus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
norepinephrine | - |
Gallus gallus | |
20 | - |
ascorbate | - |
Gallus gallus |