Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His4-tagged CYP11A1 | Bos taurus |
Crystallization (Comment) | Organism |
---|---|
CYP11A1 in complex with (22R)-22-hydroxycholesterol, sitting drop vapour diffusion methdo, mixing of 0.001 ml of 23 mg/ml protein in 50 mM potassium phosphate, pH 7.2, 20% glycerol, 0.1 M NaCl, 0.1% octyl pentaethylene glycol ether, 1 mM EDTA, and 0.05 mM (22R)-22-hydroxycholesterol, with 0.001 ml of precipitant solution containing 14% PEG 1000, 20% glycerol, 12% JEFFAMINE ED-2001, 0.1 M MES, pH 7.0, and 10% isopropyl alcohol, 18°C, overnight, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement using rat mitochondrial CYP24A1 | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(20R,22R)-20,22-dihydroxy-cholesterol + 2 reduced adrenodoxin + O2 | Bos taurus | - |
pregnenolone + 4-methylpentanal + 2 oxidized adrenodoxin + 2 H2O | - |
? | |
(22R)-22-hydroxycholesterol + 2 reduced adrenodoxin + O2 | Bos taurus | - |
(20R,22R)-20,22-dihydroxycholesterol + 2 oxidized adrenodoxin + H2O | - |
? | |
cholesterol + 2 reduced adrenodoxin + O2 | Bos taurus | - |
(22R)-22-hydroxycholesterol + 2 oxidized adrenodoxin + H2O | - |
? | |
cholesterol + 6 reduced adrenodoxin + 3 O2 | Bos taurus | overall reaction | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00189 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His4-tagged CYP11A1 by anion exchange, nickel affinity, hydroxylapatite, adrenodoxin affinity chromatography, and gel filtration | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(20R,22R)-20,22-dihydroxy-cholesterol + 2 reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + 2 oxidized adrenodoxin + 2 H2O | - |
? | |
(22R)-22-hydroxycholesterol + 2 reduced adrenodoxin + O2 | - |
Bos taurus | (20R,22R)-20,22-dihydroxycholesterol + 2 oxidized adrenodoxin + H2O | - |
? | |
(22R)-22-hydroxycholesterol + 2 reduced adrenodoxin + O2 | product binding structure analysis, detailed overview | Bos taurus | (20R,22R)-20,22-dihydroxycholesterol + 2 oxidized adrenodoxin + H2O | - |
? | |
cholesterol + 2 reduced adrenodoxin + O2 | - |
Bos taurus | (22R)-22-hydroxycholesterol + 2 oxidized adrenodoxin + H2O | - |
? | |
cholesterol + 6 reduced adrenodoxin + 3 O2 | overall reaction | Bos taurus | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cholesterol side chain cleavage enzyme | - |
Bos taurus |
CYP11A1 | - |
Bos taurus |
cytochrome P450 11A1 | - |
Bos taurus |
P450 11A1 | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | - |
Bos taurus | |
cytochrome P450 | - |
Bos taurus | |
heme | site of catalysis | Bos taurus |
General Information | Comment | Organism |
---|---|---|
additional information | the active site cavity in CYP11A1 represents a long curved tube that extends from the protein surface to the heme group, the site of catalysis. Shuttling of the sterol intermediates between the active site entrance and the heme group during the three-step reaction. Structural basis of the strict substrate specificity and high catalytic efficiency of the enzyme, overview | Bos taurus |