Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Runx2 | activation of CYP11A1 by the osteogenic transcription factor Runx2 may contribute to attenuation of osteoblast growth. Runx2 binds to the CYP11A1 gene promoter, CYP11A1 is a direct target of Runx2 | Homo sapiens | |
Runx2 | activation of CYP11A1 by the osteogenic transcription factor Runx2 may contribute to attenuation of osteoblast growth. Runx2 binds to the CYP11A1 gene promoter, CYP11A1 is a direct target of Runx2. Runx2 is rate limiting for CYP11A1 gene expression during differentation along the osteoblastic lineage | Mus musculus |
Cloned (Comment) | Organism |
---|---|
both full-length and an N-terminally truncated form amplified and inserted into TOPO TA PCR cloning vector and subsequently subcloned into the EcoRI restriction site of pCMV-tag2B vector to obtain CYP11A1 proteins with an N-terminal Flag tag or into pEGFP-C2 vector to yield an N-terminal GFP tag | Mus musculus |
expressed from pCMV-SPORT6 vector | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | both full length and truncated isoforms are predominantely localized in the cytoplasm | Mus musculus | 5737 | - |
mitochondrion | truncated form is nonmitochondrial | Mus musculus | 5739 | - |
nucleus | truncated form | Mus musculus | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
truncated form, Western blot analysis | Mus musculus |
32000 | - |
truncated form, Western blot analysis | Homo sapiens |
55000 | - |
full-length form, Western blot analysis | Mus musculus |
55000 | - |
full-length form, Western blot analysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
calvarium | low level | Mus musculus | - |
MDA-MB-231 cell | breast cancer cell, expresses both the 55-kDa isoform and the 32-kDa truncated isoform of CYP11A1 | Homo sapiens | - |
additional information | expression of the 32-kDa truncated isoform of CYP11A1 in HOB-04T8 cells | Homo sapiens | - |
additional information | very low level of endogenous CYP11A1 in MC3T3 cells and Runx2-null osteoprogenitor cells | Mus musculus | - |
osteoblast | - |
Homo sapiens | - |
osteoblast | expresses an approximately 32-kDa truncated isoform of CYP11A1, expression of an approximately 32-kDa truncated isoform of CYP11A1 | Mus musculus | - |
ovary | high levels in mature tissue | Mus musculus | - |
prostate | expresses the full length 55-kDa isoform of CYP11A1 | Mus musculus | - |
testis | high level, expresses the full length 55-kDa isoform of CYP11A1 | Mus musculus | - |
tibia | low level | Mus musculus | - |
Synonyms | Comment | Organism |
---|---|---|
cholesterol side-cleaving enzyme | - |
Mus musculus |
cholesterol side-cleaving enzyme | - |
Homo sapiens |
CYP11A1 | - |
Mus musculus |
CYP11A1 | - |
Homo sapiens |
P450scc | - |
Mus musculus |
P450scc | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | CYP11A1 expression is induced by Runx2in osteoblasts | up |
Mus musculus | CYP11A1 mRNA expression is dramatically induced within 2 h after introduction of Runx2 protein into Runx2-null cells. CYP11A1 gene expression is induced during osteoblastic differentiation | up |
General Information | Comment | Organism |
---|---|---|
metabolism | CYP11A1 is a Runx2-responsive gene and may represent an integral component of a biological program that is controlled by Runx2 to regulate enzymes mediating sterol/steroid metabolism | Mus musculus |
metabolism | CYP11A1 is a Runx2-responsive gene and may represent an integral component of a biological program that is controlled by Runx2 to regulate enzymes mediating sterol/steroid metabolism | Homo sapiens |
physiological function | CYP11A1 suppresses osteoblast proliferation, specific CYP11A1 knockdown with short hairpin RNA increases cell proliferation | Mus musculus |