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Literature summary for 1.14.15.6 extracted from

  • Tuckey, R.C.; Nguyen, M.N.; Slominski, A.
    Kinetics of vitamin D3 metabolism by cytochrome P450scc (CYP11A1) in phospholipid vesicles and cyclodextrin (2008), Int. J. Biochem. Cell Biol., 40, 2619-2626.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information vitamin D3 and cholesterol are metabolized with comparable efficiencies in cyclodextrin, but the Km for both show a strong dependence on the cyclodextrin concentration, decreasing with decreasing cyclodextrin Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0091
-
cholesterol in the presence of 0.45% cyclodextrin Bos taurus
0.0296
-
vitamin D3 in the presence of 0.45% cyclodextrin Bos taurus
0.067
-
20-hydroxyvitamin D3 in the presence of 0.45% cyclodextrin Bos taurus
1.18
-
cholesterol in the presence of 4.5% cyclodextrin Bos taurus
3.67
-
vitamin D3 in the presence of 4.5% cyclodextrin Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Bos taurus 16020
-
mitochondrion
-
Bos taurus 5739
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
20,23-dihydroxyvitamin D3 + reduced adrenodoxin + O2
-
Bos taurus 17,20,23-trihydroxyvitamin D3 + oxidized adrenodoxin + H2O
-
?
20-hydroxyvitamin D3 + reduced adrenodoxin + O2
-
Bos taurus 20,23-dihydroxyvitamin D3 + oxidized adrenodoxin + H2O
-
?
cholesterol + reduced adrenodoxin + O2
-
Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
?
vitamin D3 + reduced adrenodoxin + O2 is hydroxylated with lower efficiency than cholesterol Bos taurus 20-hydroxyvitamin D3 + oxidized adrenodoxin + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP11A1
-
Bos taurus
cytochrome P450scc
-
Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
cholesterol in the presence of 0.45% cyclodextrin Bos taurus
0.123
-
20-hydroxyvitamin D3 in the presence of 0.45% cyclodextrin Bos taurus
0.248
-
cholesterol in the presence of 4.5% cyclodextrin Bos taurus
0.328
-
vitamin D3 in the presence of 0.45% cyclodextrin Bos taurus
1.6
-
vitamin D3 in the presence of 4.5% cyclodextrin Bos taurus

Cofactor

Cofactor Comment Organism Structure
adrenodoxin
-
Bos taurus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.212
-
cholesterol in the presence of 4.5% cyclodextrin Bos taurus
0.435
-
vitamin D3 in the presence of 4.5% cyclodextrin Bos taurus
1.83
-
20-hydroxyvitamin D3 in the presence of 0.45% cyclodextrin Bos taurus
11.1
-
vitamin D3 in the presence of 0.45% cyclodextrin Bos taurus
11.17
-
cholesterol in the presence of 0.45% cyclodextrin Bos taurus