Protein Variants | Comment | Organism |
---|---|---|
K267Q | participation in electrostatic interaction of P-450scc with adrenodoxin | Bos taurus |
K403Q | participation in electrostatic interaction of P-450scc with adrenodoxin | Bos taurus |
K405Q | participation in electrostatic interaction of P-450scc with adrenodoxin | Bos taurus |
R426Q | participation in electrostatic interaction of P-450scc with adrenodoxin | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methoxychlor | pesticide of DDT, suicide inhibitor, competitive to cholesterol, substantial irreversible loss of activity, 5% inhibition within 5 min at 0.2 mM, decrease is suppressed by the presence of cholesterol | Bos taurus | |
additional information | inactivation different in absence and presence of cholesterol, protective effect of cholesterol as well as 20alpha-hydroxycholesterol and deoxycorticosterone against inactivation by methoxychlor | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.164 | - |
cholesterol | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Bos taurus | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | Bos taurus | - |
pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | precursor of all steroid hormones | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | precursor of all steroid hormones | ? | |
cholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
additional information | 21 amino-acid residues from Pro-8 to Arg-28 in the amino-terminus are located in or near the cholesterol-binding site, this site is in a quite different region from the adreno-ferredoxin binding site Pro374 to Iso-389 | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | - |
Bos taurus | |
heme | hemeprotein | Bos taurus | |
heme | content of 18.9 nmol/mg protein | Bos taurus | |
NADPH | NADPH-linked adrenodoxin reductase | Bos taurus |