Application | Comment | Organism |
---|---|---|
medicine | treatment of certain hormone-related pathologies | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(20S)-22-thiacholesterol | competitive inhibitor, is converted enzymatically to a more potent inhibitor, the (22S) and (22R) sulfoxides, inhibition by approximately 75% at 0.001 mM, no inactivation in absence of NADPH and O2 | Bos taurus | |
(20S,22R)-22-thiacholesterol S-oxide | competitive versus cholesterol, binds 10times more tightly than (22S) diastereomer, 25% and 44% inhibition at 0.00005 mM and 0.0001 mM, respectively, complete inhibition at 0.001 mM, no substrate for P-450 | Bos taurus | |
(20S,22S)-22-thiacholesterol S-oxide | competitive versus cholesterol, no substrate for P-450 | Bos taurus | |
additional information | mechanism-based inhibition, no inhibition with (20R)-22-thiacholesterol at 0.001 mM, inhibition by occupying the cholesterol binding pocket | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Bos taurus | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | Bos taurus | first step in biosynthesis of all steroid hormones | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | isocapraldehyde additional product | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(20S)-22-thiacholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | (20S,22R)-22-thiacholesterol S-oxide + (20S,22S)-22-thiacholesterol S-oxide | (22R)-sulfoxide preferentially formed by a factor of 4.2 to 1 over (22S)-sulfoxide | ? | |
cholesterol + 6 reduced adrenodoxin + 3 O2 | first step in biosynthesis of all steroid hormones | Bos taurus | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | isocapraldehyde additional product | ? | |
cholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
additional information | 3 high regioselective and stereospecific oxidation steps at the same heme active site | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
cholesterol | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | - |
Bos taurus | |
heme | - |
Bos taurus | |
NADPH | NADPH-linked adrenodoxin reductase | Bos taurus |